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Detection of short oligonucleotide sequences of hepatitis B virus using electrochemical DNA hybridisation biosensor

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Abstract

A novel, sensitive and selective electrochemical hybridisation biosensor was developed for the detection of the hepatitis B virus (HBV) using a manganese(II) complex as electrochemical indicator and a DNA probe-modified carbon paste electrode as the biosensor (DNA/CPE). The results showed that this complex could be accumulated electrochemically the immobilised dsDNA layer rather than in the single-stranded DNA (ssDNA) layer. On the basis of this, the manganese complex was used as an electrochemical hybridisation indicator for the detection of oligonucleotides related to HBV. The hybridisation event was evaluated on the basis of the difference between the reduction signals of the manganese(II) complex with the probe DNA prior to and post hybridisation with a target sequence using a differential pulse mode. Several factors affecting the immobilisation and hybridisation of oligonucleotides as well as the indicator’s accumulation were investigated. Experiments with a non-complementary and mismatch sequences demonstrated the good selectivity of the biosensor. Using this approach, the HBV target oligonucleotide’s sequence could be quantified over arange from 0.22 ng L−1 to 5.40 ng L−1, with a linear correlation coefficient of 0.9994 and the limit of detection of 0.07 ng L−1.

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References

  • Azizi, S. N., Ranjbar, S., Raoof, J. B., & Hamidi-Asl, E. (2013) Preparation of Ag/NaA zeolite modified carbon paste electrode as a DNA biosensor. Sensors and Actuators B: Chemical, 181, 319–325. DOI: 10.1016/j.snb.2013.02.026.

    Article  CAS  Google Scholar 

  • Caliskan, A., Erdem, A., & Karadeniz, H. (2009) Direct DNA Hybridization on the single-walled carbon nanotubes modified sensors detected by voltammetry and electrochemical impedance spectroscopy Electroanalysis, 21, 2116–2124. DOI: 10.1002/elan.200904640.

    Article  CAS  Google Scholar 

  • Castañeda, M. T., Merkoçi, A., Pumera, M., & Alegret, S. (2007) Electrochemical genosensors for biomedical applications based on gold nanoparticles. Biosensors and Bioelectronics, 22, 1961–1967. DOI: 10.1016/j.bios.2006.08.031.

    Article  Google Scholar 

  • Ding, C. F., Zhao, F., Zhang, M. L., & Zhang, S. (2008) Hybridization biosensor using 2,9-dimethyl-1,10-phenantroline cobalt as electrochemical indicator for detection of hepatitis B virus DNA. Bioelectrochemistry, 72, 28–33. DOI: 10.1016/j.bioelechem.2007.11.001.

    Article  CAS  Google Scholar 

  • Du, D. X., Guo, S., Tang, L., Ning, Y., Yao, Q. F., & Zhang, G. J. (2013) Graphene-modified electrode for DNA detection via PNA-DNA hybridization. Sensors and Actuators B: Chemical, 186, 563–570. DOI: 10.1016/j.snb.2013.06.045.

    Article  CAS  Google Scholar 

  • Erdem, A., Muti, M., Karadeniz, H., Congur, G., & Canavar, E. (2012) Electrochemical monitoring of indicator-free DNA hybridization by carbon nanotubes-chitosan modified disposable graphite sensors. Colloids and Surfaces B: Biointerfaces, 95, 222–228. DOI: 10.1016/j.colsurfb.2012.02.042.

    Article  CAS  Google Scholar 

  • Fojta, M., Doffková, R., & Paleček, E. (1996) Determination of traces of RNA in submicrogram amounts of single- or double-stranded DNAs by means of nucleic acid-modified electrodes. Electroanalysis, 8, 420–426. DOI: 10.1002/elan.1140080504.

    Article  CAS  Google Scholar 

  • Girousi, S., & Kinigopoulou, V. (2010) Detection of short oligonucleotide sequences using an electrochemical DNA hybridization biosensor. Central European Journal of Chemistry, 8, 732–736. DOI: 10.2478/s11532-010-0056-5.

    CAS  Google Scholar 

  • Girousi, S., Karastogianni, S., & Serpi, C. (2012) Electrochemical techniques as promising analytical tools in the DNA electrochemistry (A review). Sensing in Electroanalysis, 7, 107–140.

    Google Scholar 

  • Guo, M. D., Li, Y. Q., Guo, H.X., Wu, X.Q., & Fan, L. F. (2007) Electrochemical detection of short sequences related to the hepatitis B virus using MB on chitosan-modified CPE. Bioelectrochemistry, 70, 245–249. DOI: 10.1016/j.bioelechem.2006.09.002.

    Article  CAS  Google Scholar 

  • Hanaee, H., Ghourchian, H., & Ziaee, A. A. (2007) Nano-particle-based electrochemical detection of hepatitis B virus using stripping chronopotentiometry. Analytical Biochemistry, 370, 195–200. DOI: 10.1016/j.ab.2007.06.029.

    Article  CAS  Google Scholar 

  • Hassen, W. M., Chaix, C., Abdelghani, A., Bessueille, F., Leonard, D., & Jaffrezic-Renault, N. (2008) An impedimetric DNA sensor based on functionalized magnetic nanoparticles for HIV and HBV detection. Sensors and Actuators B: Chemical, 134, 755–760. DOI: 10.1016/j.snb.2008.06.020.

    Article  CAS  Google Scholar 

  • Jilbert, A. R. (2000) In situ hybridization protocols for detection of viral DNA using radioactive and nonradioactive DNA probes. Methods in Molecular Biology, 123, 177–193. DOI: 10.1385/1-59259-677-0:177.

    CAS  Google Scholar 

  • Kara, P., Cavdar, S., Meric, B., Erensoy, S., & Ozsoz, M. (2007) Electrochemical probe DNA design in PCR amplicon sequence for the optimum detection of microbiological diseases. Bioelectrochemistry, 71, 204–210. DOI: 10.1016/j.bioelechem.2007.05.001.

    Article  CAS  Google Scholar 

  • Karadeniz, H., Erdem, A., & Caliskan, A. (2008) Electrochemical monitoring of DNA hybridization by multiwalled carbon nanotube based screen printed electrodes. Electroanalysis, 20, 1932–1938. DOI: 10.1002/elan.200804270.

    Article  CAS  Google Scholar 

  • Karastogianni, S., Dendrinou-Samara, C., Ioannou, E., Raptopoulou, C. P., Hadjipavlou-Litina, D., & Girousi, S. (2013) Synthesis, characterization, DNA binding properties and antioxidant activity of a manganese(II) complex with NO6 chromophore. Journal of Inorganic Biochemistry, 118, 48–58. DOI: 10.1016/j.jinorgbio.2012.09.015.

    Article  CAS  Google Scholar 

  • Lee, W. M. (1997) Hepatitis B virus infection. New England Journal of Medicine, 337, 1733–1745. DOI: 10.1056/nejm199712113372406.

    Article  CAS  Google Scholar 

  • Li, X. M., Ju, H. Q., Ding, C. F., & Zhang, S. S. (2007a) Nucleic acid biosensor for detection of hepatitis B virus using 2,9-dimethyl-1,10-phenanthroline copper complex as electrochemical indicator. Analytica Chimica Acta, 582, 158–163. DOI: 10.1016/j.aca.2006.09.004.

    Article  CAS  Google Scholar 

  • Li, X. M., Ju, H. Q., Du, L. P., & Zhang, S. S. (2007b) A nucleic acid biosensor for the detection of a short sequence related to the hepatitis B virus using bis(benzimidazole)cadmium(II) dinitrate as an electrochemical indicator. Journal of Inorganic Biochemistry, 101, 1165–1171. DOI: 10.1016/j.jinorgbio.2007.05.003.

    Article  CAS  Google Scholar 

  • Li, G. J., Liu, N., Liu, S. F., & Zhang, S. H. (2008) Electrochemical biosensor based on the interaction between copper(II) complex with 4,5-diazafluorene-9-one and bromine ligands and deoxyribonucleic acid. Electrochimica Acta, 53, 2870–2876. DOI: 10.1016/j.electacta.2007.10.079.

    Article  CAS  Google Scholar 

  • Liu, N., Li, G. J., Liu, S. F., & Zhang, S. S. (2008) Electrochemical DNA biosensor for the detection of interaction between di[azino-di(5,6-azafluorene)-κ2-N,N′]dichlormanganous and DNA. Sensors and Actuators B: Chemical, 133, 582–587. DOI: 10.1016/j.snb.2008.03.026.

    Article  CAS  Google Scholar 

  • Ly, S. Y., & Chob, N. S. (2009) Diagnosis of human hepatitis B virus in non-treated blood by the bovine IgG DNA-linked carbon nanotube biosensor. Journal of Clinical Virology, 44, 43–47. DOI: 10.1016/j.jcv.2008.09.005.

    Article  CAS  Google Scholar 

  • Moriya, T., Kuramoto, I. K., Yoshizawa, H., & Holland, P. V. (2002) Distribution of hepatitis B virus genotypes among American blood donors determined with a PreS2 epitope enzyme-linked immunosorbent assay kit. Journal of Clinical Microbiology, 40, 877–880. DOI: 10.1128/jcm.40.3.877-880.2002.

    Article  Google Scholar 

  • Muti, M., Sharma, S., Erdem, A., & Papakonstantinou, P. (2011) Electrochemical monitoring of nucleic acid hybridization by single-use graphene oxide-based sensor. Electroanalysis, 23, 272–279. DOI: 10.1002/elan.201000425.

    Article  CAS  Google Scholar 

  • Niu, S. Y., Han, B., Cao, W., & Zhang, S. S. (2009) Sensitive DNA biosensor improved by luteolin copper(II) as indicator based on silver nanoparticles and carbon nanotubes modified electrode. Analytica Chimica Acta, 651, 42–47. DOI: 10.1016/j.aca.2009.08.002.

    Article  CAS  Google Scholar 

  • Paleček, E., Tomschik, M., Staňková, V., & Havran, L. (1997) Chronopotentiometric stripping of DNA at mercury electrodes. Electroanalysis, 9, 990–997. DOI: 10.1002/elan.1140091305.

    Article  Google Scholar 

  • Park, J.H., Cho, E.W., Lee, D.G., Park, J.M., Lee, Y.J., Choi, E. A., & Kim, K. L. (2000) Receptor — mediated endocytosis of hepatitis B virus PreS1 protein in EBV-transformed B-cell line. Journal of Microbiology and Biotechnology, 10, 844–850.

    CAS  Google Scholar 

  • Peterson, A. W., Heaton, R. J., & Georgiadis, R. M. (2001) The effect of surface probe density on DNA hybridization. Nucleic Acids Research, 29, 5163–5168. DOI: 10.1093/nar/29.24.5163.

    Article  CAS  Google Scholar 

  • Rice, M. E., Galus, Z., & Adams, R. N. (1983) Graphite paste electrodes: Effects of paste composition and surface states on electron-transfer rates. Journal of Electroanalytical Chemistry and Interfacial Electrochemistry, 143, 89–102. DOI: 10.1016/s0022-0728(83)80256-3.

    Article  CAS  Google Scholar 

  • Siddiquee, S., Yusof, N. A., Salleh, A. B., Bakar, F. A., & Heng, L. Y. (2010a) Electrochemical DNA biosensor for the detection of specific gene related to Trichoderma harzianum species. Bioelectrochemistry, 79, 31–36. DOI: 10.1016/j.bioelechem.2009.10.004.

    Article  CAS  Google Scholar 

  • Siddiquee, S., Yusof, N. A., Salleh, A. B., Tan, S. G., Bakar, F. A., & Heng, L. U. (2010b) DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode. Sensors and Actuators B: Chemical, 147, 198–205. DOI: 10.1016/j.snb.2010.02.014.

    Article  CAS  Google Scholar 

  • Tabassum, S. Parveen, S., & Arjimand, F. (2005) New modulated metallic macrocycles: Electrochemistry and their interaction with calf thymus DNA. Acta Biomaterialia, 1, 677–689. DOI: 10.1016/j.actbio.2005.07.002.

    Article  Google Scholar 

  • Wright, T. L., & Lau, J. Y. N. (1993) Clinical aspects of hepatitis B virus infection. The Lancet, 342, 1340–1345. DOI: 10.1016/0140-6736(93)92250-w.

    Article  CAS  Google Scholar 

  • Yola, M. L., Eren, T., & Atar, N. (2014) A novel and sensitive electrochemical DNA biosensor based on Fe@Au nanoparticles decorated graphene oxide. Electrochimica Acta, 125, 38–47. DOI: 10.1016/j.electacta.2014.01.074.

    Article  CAS  Google Scholar 

  • Young, K.C., Chang, T.T., Hsiao, W.C., Cheng, P.N., Chen, S. H., & Jen, C. M. (2002) A reverse-transcription competitive PCR assay based on chemiluminescence hybridization for detection and quantification of hepatitis C virus RNA. Journal of Virological Methods, 103, 27–39. DOI: 10.1016/s0166-0934(01)00403-7.

    Article  CAS  Google Scholar 

  • Zhang, S. S., Tan, Q. Q., Li, F., & Zhang, X. R. (2007) Hybridization biosensor using diaquabis[N-(2-pyridinylmethyl) benzamide-κ2N, O]-cadmium(II) dinitrate as a new electroactive indicator for detection of human hepatitis B virus DNA. Sensors Actuators B: Chemical, 124, 290–296. DOI: 10.1016/j.snb.2006.12.040.

    Article  CAS  Google Scholar 

  • Zhao, H. T., & Ju, H. X. (2004) Biosensor for hepatitis B virus DNA PCR product and electrochemical study of the interaction of di(2,2′-bipyridine) osmium(III) with DNA. Electroanalysis, 16, 1642–1646. DOI: 10.1002/elan.200303013.

    Article  CAS  Google Scholar 

  • Ziółkowski, R., Olejniczak, A. B., Górski, L., Janusik, J., Leśnikowski, Z. J., & Malinowska, E. (2012) Electrochemical detection of DNA hybridization using metallacarborane unit. Bioelectrochemistry, 87, 78–83. DOI: 10.1016/j.bioelechem.2011.10.005.

    Article  Google Scholar 

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  1. Department of Analytical Chemistry, Aristotle University of Thessaloniki, Panepistimioupoli, Thessaloniki, 54124, Greece

    Sophia Karastogianni & Stella Girousi

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  1. Sophia Karastogianni
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  2. Stella Girousi
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Correspondence to Stella Girousi.

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Karastogianni, S., Girousi, S. Detection of short oligonucleotide sequences of hepatitis B virus using electrochemical DNA hybridisation biosensor. Chem. Pap. 69, 202–210 (2015). https://doi.org/10.2478/s11696-014-0599-6

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  • DOI: https://doi.org/10.2478/s11696-014-0599-6

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