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Use of green fluorescent proteins for in vitro biosensing

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Abstract

Due to the considerable stability of green fluorescent proteins and their capacity to be readily permutated or mutated, they may be exploited in multiple ways to enhance the functionality of in vitro biosensors. Many possibilities, such as the formation of chimeras with other proteins or antibodies, as well as Förster resonance emission transfer performance, may be used for the highly sensitive and specific detection of the target molecules. This review considers the great potential of green fluorescent proteins as the fluorescent probing or recognition biomolecule in various in vitro biosensors applications, as well as obstacles associated with their use.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic

    Zbynek Heger, Ondrej Zitka, Rene Kizek & Vojtech Adam

  2. Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00, Brno, Czech Republic

    Zbynek Heger, Ondrej Zitka, Zdenka Fohlerova, Miguel Angel Merlos Rodrigo, Jaromir Hubalek, Rene Kizek & Vojtech Adam

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  1. Zbynek Heger
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  2. Ondrej Zitka
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  3. Zdenka Fohlerova
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  4. Miguel Angel Merlos Rodrigo
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  5. Jaromir Hubalek
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  6. Rene Kizek
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  7. Vojtech Adam
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Correspondence to Vojtech Adam.

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Heger, Z., Zitka, O., Fohlerova, Z. et al. Use of green fluorescent proteins for in vitro biosensing. Chem. Pap. 69, 54–61 (2015). https://doi.org/10.2478/s11696-014-0588-9

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  • DOI: https://doi.org/10.2478/s11696-014-0588-9

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