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Evaluation of antioxidants in Dong quai (Angelica sinensis) and its dietary supplements

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Abstract

The antioxidant activity (AA), total phenolic content (TPC) and total flavonoids content (TFC) in Dong quai (DQ, Angelica sinensis) raw materials and dietary supplements (DS) containing this plant were determined using the CUPRAC, FRAP and fluorescence methods. The antioxidant activity for DQ aqueous extracts revealed by CUPRAC was (1330.45 ± 1.30) μmol Trolox equivalent (TE) per 100 g of dry mass (DM), whereas the antioxidant activity as determined by FRAP was (1813.9 ± 2.0) μmol of TE per 100 g of DM. Lower values were noted for the fluorescence method than for CUPRAC and FRAP (ranging from (35.96 ± 0.3) to (304.6 ± 1.4) μmol of TE per 100 g of DM). The highest TPC values were determined for an aqueous extract of DQ ((3330.3 ± 2.3) μmol of TE per 100 g of DM), while TFC for ethanolic extracts of DQ was ((146.50 ± 0.5) mg of quercetin equivalent (QE) per 100 g of DM). Cinnamic acid, isomers of benzoic acid and derivatives of quercetin were analysed by HPLC-PDA. The ferulic acid concentration in an ethanolic extract of DQ was (21.83 ± 0.07) mg per 100 g of DM. Of the flavonols detected, rutin exhibited the highest concentration in ethanolic extract of DQ ((3.32 ± 0.13) mg of QE per 100 g of DM). Other phytochemicals (alkaloids, saponins, flavonoids, anthraquinones, tannins, steroids, etc.) were identified by phytoscreening colour reaction. The results were analysed by principal component analysis (PCA), cluster analysis and one-way ANOVA tests.

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References

  • Apak, R., Güçlü, K., Özyürek, M., & Çelik, S. E. (2008). Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay. Microchimica Acta, 160, 413–419. DOI: 10.1007/s00604-007-0777-0.

    Article  CAS  Google Scholar 

  • Circosta, C., De Pasquale, R., Palumbo, D. R., Samperi, S., & Occhiuto, F. (2006). Estrogenic activity of standardized extract of Angelica sinensis. Phytotherapy Research, 20, 665–669. DOI: 10.1002/ptr.1928.

    Article  CAS  Google Scholar 

  • Cisowski, W. (1995). Analiza fitochemiczna. Gdańsk, Poland: Akademia Medyczna w Gdańsku. (in Polish)

    Google Scholar 

  • Clifford, M. N. (2004). Diet-derived phenols in plasma and tissues and their implications for health. Planta Medica, 70, 1103–1114. DOI: 10.1055/s-2004-835835.

    Article  CAS  Google Scholar 

  • Cook, N. C., & Samman, S. (1996). Flavonoids — chemistry, metabolism, cardioprotective effects and dietary sources. The Journal of Nutritional Biochemistry, 7, 66–76. DOI: 10.1016/s0955-2863(95)00168-9.

    Article  CAS  Google Scholar 

  • Filipiak-Szok, A., Kurzawa, M., & Szłyk, E. (2012a). Determination of antioxidant capacity and content of phenols, phenolic acids and flavonols contents in Indian and European gooseberry. Chemical Papers, 66, 259–268. DOI: 10.2478/s11696-012-0151-5.

    Article  CAS  Google Scholar 

  • Filipiak-Szok, A., Piasta, A., Kurzawa, M., & Szłyk, E. (2012b). Determination of flavonols and phenolic acids in Puearia lobata (Kudzu) root by HPLC-PDA method. Current Issues in Pharmacy and Medical Sciences, 25, 278–281.

    Article  Google Scholar 

  • Ho, C. C., Kumaran, A., & Hwang, L. S. (2009). Bio-assay guided isolation and identification of anti-Alzheimer active compounds from the root of Angelica sinensis. Food Chemistry, 114, 246–252. DOI: 10.1016/j.foodchem.2008.09.046.

    Article  CAS  Google Scholar 

  • Huang, L. F., Li, B. Y., Liang, Y. Z., Guo, F. Q., & Wang, Y. L. (2004). Application of combined approach to analyze the constituents of essential oil from Dong quai. Analytical and Bioanalytical Chemistry, 378, 510–517. DOI: 10.1007/s00216- 003-2309-z.

    Article  CAS  Google Scholar 

  • Huang, S. H., Chen, C. C., Lin, C. M., & Chiang, B. H. (2008). Antioxidant and flavor properties of Angelica sinensis extracts as affected by processing. Journal of Food Composition and Analysis, 21, 402–409. DOI: 10.1016/j.jfca.2008.02.005.

    Article  CAS  Google Scholar 

  • Lafay, S., & Gil-Izquierdo, A. (2008). Bioavailability of phenolic acids. Phytochemistry Reviews, 7, 301–311. DOI: 10.1007/s11101-007-9077-x.

    Article  CAS  Google Scholar 

  • Lao, S. C., Li, S. P., Kan, K. K. W., Li, P., Wan, J. B., Wang, Y. T., Dong, T. X., & Tsim, K. W. K. (2004). Identification and quantification of 13 components in Angelica sinensis (Danggui) by gas chromatography-mass spectrometry coupled with pressurized liquid extraction. Analytica Chimica Acta, 526, 131–137. DOI: 10.1016/j.aca.2004.09.050.

    Article  CAS  Google Scholar 

  • Li, P., Li, S. P., Lao, S. C., Fu, C. M., Kan, K. K. W., & Wang, Y. T. (2006). Optimization of pressurized liquid extraction for Z-ligustilide, Z-butylidenephthalide and ferulic acid in Angelica sinensis. Journal of Pharmaceutical and Biomedical Analysis, 40, 1073–1079. DOI: 10.1016/j.jpba.2005.08.035.

    Article  CAS  Google Scholar 

  • Lu, G. H., Chan, K., Leung, K., Chan, C. L., Zhao, Z. Z., & Jiang, Z. H. (2005). Assay of free ferulic acid and total ferulic acid for quality assessment of Angelica sinensis. Journal of Chromatography A, 1068, 209–219. DOI: 10.1016/j.chroma.2005.01.082.

    Article  CAS  Google Scholar 

  • Mei, Q. B., Tao, J. Y., & Cui, B. (1991). Advances in the pharmacological studies of radix Angelica sinensis (Oliv) Diels (Chinese Danggui). Chinese Medical Journal, 104, 776–781.

    CAS  Google Scholar 

  • Novas, M. J., Jimenez, A. M., & Asuero, A. G. (2004). Determination of antioxidant activity of Canary seed infusions by chemiluminescence. Journal of Analytical Chemistry, 59, 75–77. DOI: 1061-9348/04/5901-0075.

    Article  CAS  Google Scholar 

  • Pharmacopoeia Commission (1988). Pharmacopoeia of the People’s Republic of China 2000 (English edition, pp. 106–107). Guangzhou, China: Guandong Science and Technology Press.

    Google Scholar 

  • Pharmacopoeia Commission (2000). Pharmacopoeia of the People’s Republic of China 2000 (English edition, pp. 158). Beijing, China: Chemical Industry Press.

    Google Scholar 

  • Pogačnik, L., & Ulrih, N. P. (2012) Application of optimized chemiluminescence assay for determination of the antioxidant capacity of herbal extracts. Luminescence, 27, 505–510. DOI: 10.1002/bio.1384.

    Article  Google Scholar 

  • Polskie Towarzystwo Farmaceutyczne (2002). Farmakopea Polska. (4th ed.) Warsawa, Poland: Polskie Towarzystwo Farmaceutyczne. (in Polish)

    Google Scholar 

  • Ross, I. A. (2001). Angelica sinensis. In Medicinal plants of the world (pp. 67–79). Totowa, NJ, USA: Humana Press. DOI: 10.1007/978-1-59259-237-1 5.

    Chapter  Google Scholar 

  • Senthamarai, R., Ismail, A. M., Kiurbha, T. S. V., & Balasubramanian, P. (2012). Pharmacognostical and phytochemical studies on leaves of Stephania japonica Linn. Journal of Chemical and Pharmaceutical Research, 4, 1457–1464.

    Google Scholar 

  • Shou, C., Li, J., & Liu, Z. (2011). Complementary and alternative medicine in the treatment of menopausal symptoms. Chinese Journal of Integrative Medicine, 17, 883–888. DOI: 10.1007/s11655-011-0932-7.

    Article  Google Scholar 

  • Song, G. X., Deng, C. H., Wu, D., & Hu, Y. M. (2004). Headspace solid-phase microextraction-gas chromatographic- mass spectrometric analysis of the essential oils of two traditional chinese medicines, Angelica pubescens and Angelica sinensis. Chromatographia, 59, 343–349. DOI: 10.1365/s10337-003-0175-7.

    CAS  Google Scholar 

  • Sun, Y. L., Tang, J., Gu, X. H., & Li, D. Z. (2005), Water-soluble polysaccharides from Angelica sinensis (Oliv.) Diels: Preparation, characterization and bioactivity. International Journal of Biological Macromolecules, 36, 283–289. DOI: 10.1016/j.ijbiomac.2005.07.005.

    Article  CAS  Google Scholar 

  • Szajdek, A., & Borowska, E. J. (2008). Bioactive compounds and health-promoting properties of berry fruits: a review. Plant Foods for Human Nutrition, 63, 147–156. DOI: 10.1007/s11130-008-0097-5.

    Article  CAS  Google Scholar 

  • Upton, R. (2003). American herbal pharmacopoeia and therapeutic compendium: Dang Gui root — Angelica sinensis (Oliv.) Diels. Scotts Valley, CA, USA: American Herbal Pharmacopoeia.

    Google Scholar 

  • Wang, S., Maa, H. Q., Sunc, Y. J., Qiao, C. D., Shao, S. J., & Jiang, S. X. (2007). Fingerprint quality control of Angelica sinensis (Oliv.) Diels by high-performance liquid chromatography coupled with discriminant analysis. Talanta, 72, 434–436. DOI: 10.1016/j.talanta.2006.11.006.

    Article  CAS  Google Scholar 

  • Weng, C. J., & Yen, G. C. (2012). Chemopreventive effects of dietary phytochemicals against cancer invasion and metastasis: Phenolic acids, monophenol, polyphenol, and their derivatives. Cancer Treatment Reviews, 38, 76–87. DOI: 10.1016/j.ctrv.2011.03.001.

    Article  CAS  Google Scholar 

  • Wojcikowski, K., Wohlmuth, H., Johnson, D. W., Rolfe, M., & Gobe, G. (2009). An in vitro investigation of herbs traditionally used for kidney and urinary system disorders: Potential therapeutic and toxic effects. Nephrology, 14, 70–79. DOI: 10.1111/j.1440-1797.2008.01017.x.

    Article  Google Scholar 

  • Zhao, K. J., Dong, T. T. X., Tu, P. F., Song, Z. H., Lo, C. K., & Tsim, K. W. K. (2003). Molecular genetic and chemical assessment of radix Angelica (Danggui) in China. Journal of Agricultural and Food Chemistry, 51, 2576–2583. DOI: 10.1021/jf026178h.

    Article  CAS  Google Scholar 

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  1. Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7 St., 87-100, Toruń, Poland

    Anna Filipiak-Szok, Marzanna Kurzawa & Edward Szłyk

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  1. Anna Filipiak-Szok
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  2. Marzanna Kurzawa
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  3. Edward Szłyk
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Correspondence to Anna Filipiak-Szok.

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Filipiak-Szok, A., Kurzawa, M. & Szłyk, E. Evaluation of antioxidants in Dong quai (Angelica sinensis) and its dietary supplements. Chem. Pap. 68, 493–503 (2014). https://doi.org/10.2478/s11696-013-0485-7

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