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Trichinella spiralis reinfection: changes in cellular and humoral immune response in BALB/c mice

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Helminthologia

Summary

Trichinella spiralis infection induces a host cell-mediated and humoral response. The role of T and B lymphocytes in the immune response of mice reinfected with 2 × 400 T. spiralis larvae was studied in relation to the parasite burden. BALB/c mice were infected on days 0 and 60 and immunological parameters were examined within a period of 180 days. In comparison with a single T. spiralis infection, T- and B-lymphocytes in reinfected mice responded by a significant increase in the proliferative activity during 10 days after reinfection. At the same time, the percentages of CD4+ T-cells of reinfected mice were also increased. In contrast, the CD8+ T-cell numbers were significantly reduced almost 30 days after reinfection. High concentration of serum IFN-γ lasted till the end of the experiment. The IL-5 level was increased only for 2 weeks after reinfection, followed by its decrease. Kinetics of specific anti-Trichinella immunoglobulins IgG2a was not affected with reinfection, but specific antibodies IgG1 significantly increased after reinfection and persisted elevated till the end of the experiment. Lower numbers of adults (69.2 % reduction) in the small intestine and 72.3 % reduction in muscle larvae were found after reinfection. Stimulation of the host immune response — the increased activity of CD4+ T lymphocytes and high levels of IFN-γ and specific IgG1 after reinfection, contributed to the reduction of the parasite burden.

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Authors and Affiliations

  1. Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovak Republic

    E. Dvorožňáková & Z. Hurníková

  2. Mammal Research Institute, Polish Academy of Sciences, Waszkiewicza 1, 17-230, Białowieża, Poland

    M. Kołodziej-Sobocińska

  3. W. Stefański Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818, Warsaw, Poland

    M. Kołodziej-Sobocińska

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  1. E. Dvorožňáková
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  2. M. Kołodziej-Sobocińska
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  3. Z. Hurníková
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Correspondence to E. Dvorožňáková.

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Dvorožňáková, E., Kołodziej-Sobocińska, M. & Hurníková, Z. Trichinella spiralis reinfection: changes in cellular and humoral immune response in BALB/c mice. Helminthologia 49, 201–210 (2012). https://doi.org/10.2478/s11687-012-0039-5

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  • DOI: https://doi.org/10.2478/s11687-012-0039-5

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