Skip to main content
SpringerLink
Log in

Excretory/secretory products of Fasciola hepatica but not recombinant phosphoglycerate kinase induce death of human hepatocyte cells

  • Research Note
  • Published:
Acta Parasitologica Aims and scope Submit manuscript

Abstract

The liver fluke Fasciola hepatica infects a wide range of hosts, and has a considerable impact on the agriculture industry, mainly through infections of sheep and cattle. Further, human infection is now considered of public health importance and is hyperendemic in some regions. The fluke infection causes considerable damage to the hosts’ liver. However, the mechanisms of liver destruction have not yet been completely elucidated. In the present report we incubated a human liver cell line in the presence of either F. hepatica excretory/secretory material (FhES) or recombinant phosphoglycerate kinase (FhPGK). Dosedependent cytotoxicity in the presence of FhES was observed, indicating that FhES is capable of killing human hepatocytes, supporting a role for FhES in damaging host liver cells during infection; while treatment with a recombinant intracellular protein — FhPGK, had no impact on cell survival.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Behm C.A., Sangster N.C. 1999. Pathology, pathophysiology and clinical aspects. In: (Ed. J.P. Dalton) Fasciolosis. CABI Publishing, Wallingford, 185–224.

    Google Scholar 

  • Berasain P., Goni F., McGonigle S., Dowd A., Dalton J.P., Frangione B., Carmona C. 1997. Proteinases secreted by Fasciola hepatica degrade extracellular matrix and basement membrane components. Journal of Parasitology, 83, 1–5.

    Article  PubMed  CAS  Google Scholar 

  • Donnelly S., O’Neill S.M., Sekiya M., Mulcahy G., Dalton J.P. 2005. Thioredoxin peroxidase secreted by Fasciola hepatica induces the alternative activation of macrophages. Infection and Immunity, 73, 166–173. DOI: 10.1128/IAI.73.1.166-173.2005.

    Article  PubMed  CAS  Google Scholar 

  • Flynn R.J., Irwin J.A., Olivier M., Sekiya M., Dalton J.P., Mulcahy G. 2007. Alternative activation of ruminant macrophages by Fasciola hepatica. Veterinary Immunology and Immunopathology, 120, 31–40. DOI: 10.1016/j.vetimm.2007.07.003.

    Article  PubMed  CAS  Google Scholar 

  • Gajewska A., Smaga-Kozlowska K., Kotomski G. 2006. Effect of excretory-secretory products of Fasciola hepatica on the functioning of rat hepatocytes. Medycyna Weterynaryjna, 62, 459–462.

    Google Scholar 

  • Guasconi L., Serradell M.C., Masih D.T. 2012. Fasciola hepatica products induce apoptosis of peritoneal macrophages. Veterinary Immunology and Immunopathology, 148, 359–363. DOI:10.1016/j.vetimm.2012.06.022.

    Article  PubMed  CAS  Google Scholar 

  • Hamilton C.M., Dowling D.J., Loscher C.E., Morphew R.M., Brophy P.M., O’Neill S.M. 2009. The Fasciola hepatica tegumental antigen suppresses dendritic cell maturation and function. Infection and Immunity, 77, 2488–2498. DOI: 10.1128/IAI.00919-08.

    Article  PubMed  CAS  Google Scholar 

  • Jaros S., Jaros D., Wesolowska A., Zygner W., Wedrychowicz H. 2010. Blocking Fasciola hepatica’s energy metabolism — a pilot study of vaccine potential of a novel gene — phosphoglycerate kinase. Veterinary Parasitology, 172, 229–237. DOI: 10.1016/j.vetpar.2010.05.008.

    Article  PubMed  CAS  Google Scholar 

  • Jefferies J.R., Campbell A.M., van Rossum A.J., Barrett J., Brophy P.M. 2001. Proteomic analysis of Fasciola hepatica excretory-secretory products. Proteomics, 1, 1128–1132. DOI: 10.1002/1615-9861(200109)1:9〈1128::AID-PROT1128〉3.0.CO;2-0.

    Article  PubMed  CAS  Google Scholar 

  • Mas-Coma S. 2005. Epidemiology of fascioliasis in human endemic areas. Journal of Helminthology, 79, 207–216. DOI: 10.1079/JOH2005296.

    Article  PubMed  CAS  Google Scholar 

  • Morphew R.M., Wright H.A., LaCourse E.J., Woods D.J., Brophy P.M. 2007. Comparative proteomics of excretory-secretory proteins released by the liver fluke Fasciola hepatica in sheep host bile and during in vitro culture ex host. Molecular & Cellular Proteomics: MCP, 6, 963–972. DOI: 10.1074/mcp.M600375-MCP200.

    Article  CAS  Google Scholar 

  • Norbury L.J., Beckham S., Pike R.N., Grams R., Spithill T.W., Fecondo J.V., Smooker P.M. 2011. Adult and juvenile Fasciola cathepsin L proteases: different enzymes for different roles. Biochimie, 93, 604–611. DOI: 10.1016/j.biochi.2010.12.004.

    Article  PubMed  CAS  Google Scholar 

  • Robinson M.W., Menon R., Donnelly S.M., Dalton J.P., Ranganathan S. 2009. An integrated transcriptomics and proteomics analysis of the secretome of the helminth pathogen Fasciola hepatica: proteins associated with invasion and infection of the mammalian host. Molecular & Cellular Proteomics: MCP, 8, 1891–1907. DOI: 10.1074/mcp.M900045-MCP200.

    Article  CAS  Google Scholar 

  • Serradell M.C., Guasconi L., Masih D.T. 2009. Involvement of a mitochondrial pathway and key role of hydrogen peroxide during eosinophil apoptosis induced by excretory-secretory products from Fasciola hepatica. Molecular and Biochemical Parasitology, 163, 95–106. DOI: 10.1016/j.molbiopara.2008.10.005.

    Article  PubMed  CAS  Google Scholar 

  • Wesołowska A., Gajewska A., Smaga-Kozłowska K., Kotomski G., Wędrychowicz H. 2012. Effect of Fasciola hepatica proteins on the functioning of rat hepatocytes. Parasitology Research, 110, 395–402. DOI: 10.1007/s00436-011-2504-3.

    Article  PubMed  Google Scholar 

  • WHO. 1995. Control of foodborne trematode infections: report of a WHO study group. World Health Organization technical report series, 849, 1–157.

    Google Scholar 

  • Wilson R.A., Wright J.M., de Castro-Borges W., Parker-Manuel S.J., Dowle A.A., Ashton P.D., Young N.D., Gasser R.B., Spithill T.W. 2011. Exploring the Fasciola hepatica tegument proteome. International Journal for Parasitology, 41, 1347–1359. DOI: DOI 10.1016/j.ijpara.2011.08.003.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences — SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland

    Piotr Bąska

  2. Witold Stefański Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818, Warsaw, Poland

    Luke J. Norbury, Kamil Januszkiewicz & Halina Wędrychowicz

  3. Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences — SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland

    Marcin Wiśniewski & Halina Wędrychowicz

  4. Central Forensic Laboratory of the Police, 7 Aleje Ujazdowskie Street, 00-583, Warsaw, Poland

    Kamil Januszkiewicz

Authors
  1. Piotr Bąska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Luke J. Norbury
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marcin Wiśniewski
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kamil Januszkiewicz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Halina Wędrychowicz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Piotr Bąska.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bąska, P., Norbury, L.J., Wiśniewski, M. et al. Excretory/secretory products of Fasciola hepatica but not recombinant phosphoglycerate kinase induce death of human hepatocyte cells. Acta Parasit. 58, 215–217 (2013). https://doi.org/10.2478/s11686-013-0126-x

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11686-013-0126-x

Keywords

Search

Navigation