Abstract
Dynamic instability is an essential phenomenon in eukaryotic nuclear division and prokaryotic plasmid R1 segregation. Although the molecular machines used in both systems differ greatly in composition, strong similarities and requisite nuances in dynamics and segregation mechanisms are observed. This brief examination of the current literature provides a functional comparison between prokaryotic and eukaryotic dynamically unstable filaments, specifically ParM and microtubules. Additionally, this mini-review should support the notion that any dynamically unstable filament could serve as the molecular machine driving DNA segregation, but these machines possess auxiliary features to adapt to temporal and spatial disparities in either system.
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Abbreviations
- APC:
-
anaphase-promoting complex
- ATP:
-
adenosine-5′-triphosphate
- CDK:
-
cyclin-dependent kinase E. coli-Escherichia coli
- GTP:
-
guanosine-5′-triphosphate
- MT:
-
microtubule
References
Howard, J. and Hyman, A.A. Dynamics and mechanics of the microtubule plus end. Nature 422 (2003) 753–758.
Tolic-Norrelykke, I.M. Push-me-pull-you: how microtubules organize the cell interior. Eur. Biophys. J. Biophys. Lett. 37 (2008) 1271–1278.
Garner, E.C., Campbell, C.S., We ibel, D.B. and Mullins, R.D. Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog. Science 315 (2007) 1270–1274.
Blohm, D. and Goebel, W. Restriction map of the antibiotic resistance plasmid R1drd-19 and its derivatives pKN102 (R1drd-19B2) and R1drd-16 for the enzymes BamHI, HindIII, EcoRI and SalI. Mol. Gen. Genet. 167 (1978) 119–127.
Erickson, H.P. Evolution of the cytoskeleton. Bioessays 29 (2007) 668–677.
Dogterom, M., Kerssemakers, J.W., Romet-Lemonne, G. and Janson, M.E. Force generation by dynamic microtubules. Curr. Opin. Cell Biol. 17 (2005) 67–74.
Sanhaji, M., Friel, C.T., Kreis, N.N., Kramer, A., Martin, C., Howard, J., Strebhardt, K. and Yuan, J. Functional and spatial regulation of mitotic centromere-associated kinesin by cyclin-dependent kinase 1. Mol. Cell. Biol. 30 (2010) 2594–2607.
Chee, M.K. and Haase, S.B. B-cyclin/CDKs regulate mitotic spindle assembly by phosphorylating kinesins-5 in budding yeast. PLoS Genet. 6 (2010) e1000935.
Peters, J.M. The anaphase-promoting complex: proteolysis in mitosis and beyond. Mol. Cell 9 (2002) 931–943.
Westermann, S., Wang, H.W., Avila-Sakar, A., Drubin, D.G., Nogales, E. and Barnes, G. The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440 (2006) 565–569.
Welburn, J.P., Grishchuk, E.L., Backer, C.B., Wilson-Kubalek, E.M., Yates, J.R., 3rd and Cheeseman, I.M. The human kinetochore Ska1 complex facilitates microtubule depolymerization-coupled motility. Dev. Cell 16 (2009) 374–385.
Holy, T.E. and Leibler, S. Dynamic instability of microtubules as an efficient way to search in space. Proc. Natl. Acad. Sci. U.S.A. 91 (1994) 5682–5685.
Salje, J., Zuber, B. and Lowe, J. Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation. Science 323 (2009) 509–512.
Jun, S. and Wright, A. Entropy as the driver of chromosome segregation. Nat. Rev. Microbiol. 8 (2010) 600–607.
Gerdes, K., Howard, M. and Szardenings, F. Pushing and pulling in prokaryotic DNA segregation. Cell 141 (2010) 927–942.
Garner, E.C., Campbell, C.S. and Mullins, R.D. Dynamic instability in a DNA-segregating prokaryotic actin homolog. Science 306 (2004) 1021–1025.
Zhang, D., Rogers, G.C., Buster, D.W. and Sharp, D.J. Three microtubule severing enzymes contribute to the “Pacman-flux” machinery that moves chromosomes. J. Cell Biol. 177 (2007) 231–242.
Castagnetti, S., Oliferenko, S. and Nurse, P. Fission yeast cells undergo nuclear division in the absence of spindle microtubules. PLoS Biol. 8 (2010) e1000512.
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Fuesler, J.A., Li, HJ.S. Dynamic instability — A common denominator in prokaryotic and eukaryotic DNA segregation and cell division. Cell Mol Biol Lett 17, 542–548 (2012). https://doi.org/10.2478/s11658-012-0026-3
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DOI: https://doi.org/10.2478/s11658-012-0026-3