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DREAM regulates insulin promoter activity through newly identified DRE element

  • Research Article
  • Published:
Central European Journal of Biology

Abstract

Downstream regulatory element antagonist modulator (DREAM) protein is a 31 kDa Ca2+-regulated transcriptional repressor. It functions as a silencer of the gene transcription. In low intracellular free Ca2+ concentration DREAM tightly binds to the downstream regulatory element (DRE) of gene promoter and impedes the transcription. In higher Ca2+ concentrations DREAM binds Ca2+ and disconnects from DRE of the gene promoter enabling transcription. We report that DREAM is expressed in different human tissues including the pancreas, where it is located in the islets of Langerhans. Location of DREAM in RIN-F5 cells in cultures is restricted to the nucleus and membranes and changes after increased Ca2+-levels. The proteins dissociate from dimmers to monomers and translocate out of the nucleus. The expression of DREAM in β-cells in the islets of Langerhans regulates the promoter activity of the insulin gene by directly interacting with the sequence located between +52 bp and +81 bp downstream of the transcriptional start site of the promoter. Our results provide evidence for the existence of DRE sequence in the insulin gene promoter. It is suggested that DREAM is a repressor of insulin gene transcription, whose effect is mediated by direct binding to DRE sequence.

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Authors and Affiliations

  1. Department of Immunobiology of Reproduction, Institute of Biology and Immunology of Reproduction to Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    Teodora Daneva, Shina Pashova & Volodia Georgiev

  2. Department of Human and Animal Physiology, Faculty of Biology, ‘St. Kliment Ohridski’ University of Sofia, 1164, Sofia, Bulgaria

    Radoslava Emilova & Hristo Gagov

  3. Microbiology Department, National Center of Infectious and Parasitic Diseases, 1504, Sofia, Bulgaria

    Plamen Padeshki

Authors
  1. Teodora Daneva
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  2. Shina Pashova
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  3. Radoslava Emilova
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  4. Plamen Padeshki
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  5. Hristo Gagov
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  6. Volodia Georgiev
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Correspondence to Teodora Daneva.

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Daneva, T., Pashova, S., Emilova, R. et al. DREAM regulates insulin promoter activity through newly identified DRE element. cent.eur.j.biol. 8, 97–106 (2013). https://doi.org/10.2478/s11535-013-0123-3

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  • DOI: https://doi.org/10.2478/s11535-013-0123-3

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