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Polar constituents of Ligustrum vulgare L. and their effect on lipoxygenase activity

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Abstract

The present work summarizes results of isolation and identification of polar constituents of the methanolic extract of Ligustrum vulgare L. leaves and of the evaluation of inhibiting activity of selected isolates on rat lung cytosol fraction lipoxygenase. Six different compounds were isolated from the ethylacetate and butanol portions of the methanolic extract (hydroxytyrosol and its glucoside, ligustroflavon, oleuropein, acteoside, echinacoside). The inhibitory activity of oleuropein, echinacoside and the water infusion of Ligustrum vulgare leaves tested on LOX was expressed as IC50. Kinetic parameters (K M, V max) and type of inhibition were determined. As the most effective in competitive inhibition of LOX, oleuropein was proved.

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Authors and Affiliations

  1. Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32, Bratislava, Slovakia

    Pavel Mučaji, Milan Nagy & Anna Záhradníková

  2. Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32, Bratislava, Slovakia

    Ivana Holková & Lýdia Bezáková

  3. Institute of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého 1/3, 612 42, Brno, Czech Republic

    Emil Švajdlenka

  4. Department of NMR and Mass Spectroscopy, Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia

    Tibor Liptaj & Nadežda Prónayová

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  1. Pavel Mučaji
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  2. Milan Nagy
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  3. Anna Záhradníková
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  4. Ivana Holková
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  5. Lýdia Bezáková
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  6. Emil Švajdlenka
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  7. Tibor Liptaj
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Correspondence to Pavel Mučaji.

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Mučaji, P., Nagy, M., Záhradníková, A. et al. Polar constituents of Ligustrum vulgare L. and their effect on lipoxygenase activity. Chem. Pap. 65, 367–372 (2011). https://doi.org/10.2478/s11696-011-0015-4

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