Skip to main content
Log in

Influence of selected fluorescent dyes on small aquatic organisms

  • Published:
Acta Geophysica Aims and scope Submit manuscript

Abstract

Rhodamine B and Rhodamine WT are fluorescent dyes commonly used as tracers in hydrological investigations. Since introducing intensely red substances into rivers raises understandable doubts of ecological nature, the authors aimed at examining the influence of these dyes on small water fauna using bioindication methods. Quantitative results, calculated with the use of Bliss-Weber probit statistical method, were achieved by means of standardized ecotoxicological tests containing ready-to-hatch resting forms of fairy shrimp (Thamnocephalus platyurus). Qualitative studies included observation of water flea crustacean (Daphnia magna) and horned planorbis snail (Planorbis corneus), both typically present in rivers and representative for temperate climate, as well as guppy fish (Poecilla reticulata), paramecium protozoan (Paramaecium caudatum) and the above-mentioned fairy shrimp. The investigation revealed that both dyes in concentrations used for hydrological purposes are low enough to exert almost no toxic impact on water fauna considered.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Behrens, H., U. Beims, H. Dieter, G. Dietze, T. Eikmann, T. Grummt, H. Hanisch, H. Henseling, W. Käss, H. Kerndorff, C. Leibundgut, U. Müller-Wegener, I. Rönnefahrt, B. Scharenberg, R. Schleyer, W. Schloz, and F. Tilkes (2001), Toxicological and ecotoxicological assessment of water tracers, Hydrogeol. J. 9, 3, 321–325, DOI: 10.1007/s100400100126.

    Article  Google Scholar 

  • Dominguez, J., J. Borroto, E. Perez, and A. Hernandez (2004), Use of 99mTcO 4 and Rhodamine WT as tracers and the mathematical convolution procedure to establish the alarm model in the Almendares River, J. Radioan. Nucl. Chem. 260, 2, 417–420, DOI: 10.1023/B:JRNC.0000027118.79238.c8.

    Article  Google Scholar 

  • Fernald, A.G., P.J. Jr. Wigington, and D.H. Landers (2001), Transient storage and hyporheic flow along the Willamette River, Oregon: Field measurements and model estimates, Water Resour. Res. 37, 6, 1681–1694, DOI: 10.1029/2000WR900338.

    Article  Google Scholar 

  • Field, M.S., R.G. Wilhelm, J.F. Quinlan, and T.J. Aley (1995), An assessment of the potential adverse properties of fluorescent tracer dyes used for groundwater tracing, Environ. Monit. Assess. 38, 1, 75–96, DOI: 10.1007/BF00547128.

    Article  Google Scholar 

  • Gooseff, M.N., J. LaNier, R. Haggerty, and K. Kokkeler (2005), Determining inchannel (dead zone) transient storage by comparing solute transport in a bedrock channel - alluvial channel sequence, Oregon, Water Resour. Res., 41, W06014, DOI: 10.1029/2004WR003513.

    Article  Google Scholar 

  • Govindarajulu, Z. (2001), Statistical Techniques in Bioassay, 2nd ed., Karger Publishers, Basel, 234 pp.

    Google Scholar 

  • Kaza, M., J. Mankiewicz-Boczek, K. Izydorczyk, and J. Sawicki (2007), Toxicity assessment of water samples from rivers in Central Poland using a battery of microbiotests - a pilot study, Polish J. Environ. Stud. 16, 1, 81–89.

    Google Scholar 

  • Lee, K.N. (1993), Compass and Gyroscope: Integrating Science and Politics for the Environment, Island Press, Washington D.C., 255 pp.

    Google Scholar 

  • Mines, C.H., A. Ghadouani, and G.N. Ivey (2009), Dying to find the source - the quantitative use of rhodamine WT as a proxy for soluble point source pollutants in closed pipe surface drainage networks, Hydrol. Earth Syst. Sci. 13, 11, 2169–2178.

    Article  Google Scholar 

  • Pepling, M., P.H. Haward, and P.R. Durkin (1997), Use and Assessment of Marker Dyes Used with Herbicydes, Syracuse Environmental Research Associates Inc., New York, Rep. SERA TR 96-21-07-03b, 47 pp.

    Google Scholar 

  • Rowiński, P.M., and J.J. Napiórkowski (eds.) (2001), Water quality issues in the Upper Narew Valley (Monographic volume), Publs. Inst. Geophys. Pol. Acad. Sc. E-2, 325, 187 pp.

  • Rowiński, P.M., J.J. Napiórkowski, and A. Owczarczyk (2003), Transport of passive admixture in a multi-channel river system - the Upper Narew case study. Part 2. Application of dye tracer method, Ecohydrol. Hydrobiol. 3, 4, 381–388.

    Google Scholar 

  • Rowiński, P.M., I. Guymer, and K. Kwiatkowski (2008), Response to the slug injection of a tracer - a large-scale experiment in a natural river, Hydrolog. Sci. J. 53, 6, 1300–1309, DOI: 10.1623/hysj.53.6.1300.

    Article  Google Scholar 

  • Sargaonkar, A., A. Kumar, and P. Khanna (2003), Dispersion study for two-dimensional modelling. Int. J. Environ. Stud. 60, 6, 537–546, DOI: 10.1080/0020723032000050385.

    Article  Google Scholar 

  • Shucksmith, J., J. Boxall, and I. Guymer (2007), Importance of advective zone in longitudinal mixing experiments, Acta Geophys. 55, 1, 95–103, DOI: 10.2478/s11600-006-0042-7.

    Article  Google Scholar 

  • Smart, P.L., and I.M.S. Laidlaw (1977), An evaluation of some fluorescent dyes for water tracing, Water Resour. Res. 13, 1, 15–33, DOI: 10.1029/WR013i001p00015.

    Article  Google Scholar 

  • Sosak-Świderska, B., D. Tyrawska, and D. Dzido (1998), Daphnia magna ecotoxicity test with parathion, Chemosphere 37, 14–15, 2989–3000, DOI: 10.1016/S0045-6535(98)00339-7.

    Article  Google Scholar 

  • Sutton, D.J., Z.J. Kabala, A. Francisco, and D. Vasudevan (2001), Limitations and potential of commercially available rhodamine WT as a groundwater tracer, Water Resour. Res., 37, 6, 1641–1656, DOI: 10.1029/2000WR900295.

    Article  Google Scholar 

  • Thamnotoxkit FTM (1995), Crustacean Toxicity Screening Test for Freshwater: Standard Operational Procedure, Creasel Ltd., Deinze, Belgium, 23 pp.

    Google Scholar 

  • Walker, C.H., S.P. Hopkin, R.M. Sibly, and D.B. Peakall (2001), Principles of Ecotoxicology, 2nd ed., Taylor and Francis Publ., London, 309 pp.

    Google Scholar 

  • Wallis, S. (2005), Experimental study of travel times in a small stream. In: W. Czernuszenko and P.M. Rowiński (eds.), Water Quality Hazards and Dispersion of Pollutants, Springer, New York, 109–120, DOI: 10.1007/0-387-23322-9_6.

    Chapter  Google Scholar 

  • Wernli, H.R. (2002), Report on tracer experiment at Lake Sarez 2002, University of Bern, Geographic Institute, Soil Section, Switzerland, 29 pp.

    Google Scholar 

  • Wilson, J.F. Jr., E.D. Cobb, and F.A. Kilpatrick (1986), Fluorometric procedures for dye tracing. In: Techniques of Water-Resources Investigations of the United States Geological Survey, Book 3. Application in Hydraulics, USGS Publ., Denver, 1–34.

    Google Scholar 

  • Wolska, L., A. Sagajdakow, A. Kuczyńska, and J. Namieśnik (2007), Application of ecotoxicological studies in integrated environmental monitoring: Possibilities and problems, Trends Anal. Chem. 26, 4, 332–344, DOI: 10.1016/j.trac.2006.11.012.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Paweł M. Rowiński.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rowiński, P.M., Chrzanowski, M.M. Influence of selected fluorescent dyes on small aquatic organisms. Acta Geophys. 59, 91–109 (2011). https://doi.org/10.2478/s11600-010-0024-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11600-010-0024-7

Key words

Navigation