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Cytostatic and cytotoxic properties of monensic acid and its biometal(II) complexes against human tumor / non-tumor cell lines

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Central European Journal of Chemistry

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Abstract

The anticancer activity of monensic acid (MonH) and its biometal(II) complexes [M(Mon)2(H2O)2](M = Mg, Ca, Mn, Co, Ni, Zn) was evaluated against cultured human permanent cell lines established from glioblastoma multiforme (8MGBA) and cancers of the lung (A549), breast (MCF-7), uterine cervix (HeLa) and liver (HepG2). The viability and proliferation of the non-tumor human embryonic cell line Lep3 was also tested. The investigations were carried out using a thiazolyl blue tetrazolium bromide test, neutral red uptake cytotoxicity assay, crystal violet staining, colony forming method and double staining with acridin orange and propidium iodide. The results obtained reveal that the compounds applied at concentrations of 0.5–25 µg mL−1 for 24–72 h decrease the viability and proliferation of the treated cells in a time- and concentration-dependent manner. The metal(II) complexes studied (especially those of Co(II), Ni(II) and Zn(II)) have been found to express stronger cytotoxic and cytostatic activities than the non-coordinated monensic acid. The non-tumor human cell line showed strong chemosensitivity towards compounds tested comparable to that of cultured human tumor cell lines.

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Authors and Affiliations

  1. Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    Radostina I. Alexandrova, Tanya Zhivkova & Marin Alexandrov

  2. Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    Georgi Miloshev & Milena Georgieva

  3. Department of Analytical Chemistry, Faculty of Chemistry and Pharmacy, Sofia University, 1164, Sofia, Bulgaria

    Ivayla N. Pantcheva & Mariana Io. Mitewa

Authors
  1. Radostina I. Alexandrova
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  2. Tanya Zhivkova
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  3. Marin Alexandrov
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  4. Georgi Miloshev
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  5. Milena Georgieva
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  6. Ivayla N. Pantcheva
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  7. Mariana Io. Mitewa
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Correspondence to Ivayla N. Pantcheva.

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Alexandrova, R.I., Zhivkova, T., Alexandrov, M. et al. Cytostatic and cytotoxic properties of monensic acid and its biometal(II) complexes against human tumor / non-tumor cell lines. cent.eur.j.chem. 10, 1464–1474 (2012). https://doi.org/10.2478/s11532-012-0071-9

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  • DOI: https://doi.org/10.2478/s11532-012-0071-9

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