Review Article


, Volume 72, Issue 11, pp 1495-1520

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Ten Years of Experience with Biphasic Insulin Aspart 30

From Drug Development to the Latest Clinical Findings
  • Andreas LieblAffiliated withFachklinik Bad Heilbrunn Email author 
  • , Vinay PrustyAffiliated withNovo Nordisk A/S
  • , Paul ValensiAffiliated withDepartment of Endocrinology Diabetology Nutrition, Paris Nord University, Jean Verdier Hospital, Assistance Publique - Hôpitaux De Paris (AP-HP), Centre De Recherche en Nutrition Humaine - Ile de France (CRNH-IdF)
  • , Ryuzo KawamoriAffiliated withDepartment of Metabolism and Endocrinology, Juntendo University School of Medicine
  • , Jens Sandahl ChristiansenAffiliated withDepartment of Endocrinology-M, Aarhus University Hospital
  • , Andrew J. PalmerAffiliated withMenzies Research Institute Tasmania, University of Tasmania
  • , Per BalschmidtAffiliated withNovo Nordisk A/S
  • , Robert LigthelmAffiliated withEHM Clinic
  • , Viswanathan MohanAffiliated withDr Mohan’s Diabetes Specialities Centre


Biphasic insulin aspart 30 (BIAsp 30) includes 30% soluble rapid-acting insulin aspart (IAsp) along with an intermediate-acting 70% protaminated IAsp that provides coverage of prandial and basal insulin in a single injection. As BIAsp 30 has been available internationally for 10 years, this review provides a comprehensive overview of the discovery of BIAsp 30, its pharmacokinetic and pharmacodynamic profile, safety and efficacy outcomes from the clinical trial programme, ‘real-life’ clinical insights provided by observational study data, and cost effectiveness and quality-of-life information. These studies have demonstrated that BIAsp 30 once or twice daily is an appropriate option for insulin initiation. BIAsp 30 also provides a switch option in patients on biphasic human insulin (BHI). Switching from BHI to BIAsp 30 is associated with improved postprandial glucose (PPG) and reduced nocturnal and major hypoglycaemia, although daytime hypoglycaemia is higher with BIAsp 30. Intensification of BIAsp 30 can be achieved by increasing the number of daily doses up to three times daily with meals. Therefore, BIAsp 30 provides an intensification option for individuals who are not achieving control with basal insulin and would prefer the simplicity of a single biphasic insulin instead of progressing to a basal-bolus approach. BIAsp 30 has a simple dose-titration algorithm, which enables patients to effectively self-titrate their insulin dose. Cost-effectiveness analyses have demonstrated that BIAsp 30 is cost effective or dominant compared with BHI 30 or insulin glargine in a number of healthcare settings. In conclusion, BIAsp 30 offers a simple and flexible option for insulin initiation and intensification that provides coverage of both fasting and prandial glucose.