Journal of Zhejiang University SCIENCE B

, Volume 11, Issue 12, pp 895–904

Improved myocardial perfusion and cardiac function by controlled-release basic fibroblast growth factor using fibrin glue in a canine infarct model

Authors

    • Department of Cardiology, Beijing Anzhen HospitalCapital Medical University
  • Wang Xiao
    • Department of Cardiology, Beijing Anzhen HospitalCapital Medical University
  • Qiao Shi-bin
    • Department of Cardiology, Beijing Anzhen HospitalCapital Medical University
  • Zeng Qiu-tang
    • Union Hospital of Tongji Medical College HuazhongUniversity of Science and Technology
  • Jiang Ju-quan
    • Union Hospital of Tongji Medical College HuazhongUniversity of Science and Technology
  • Liu Xiao-qing
    • Union Hospital of Tongji Medical College HuazhongUniversity of Science and Technology
  • Zhu Xiang-ming
    • Union Hospital of Tongji Medical College HuazhongUniversity of Science and Technology
  • Cao Guo-xiang
    • Union Hospital of Tongji Medical College HuazhongUniversity of Science and Technology
  • Ma Chang-sheng
    • Department of Cardiology, Beijing Anzhen HospitalCapital Medical University
Article

DOI: 10.1631/jzus.B1000302

Cite this article as:
Nie, S., Xiao, W., Shi-bin, Q. et al. J. Zhejiang Univ. Sci. B (2010) 11: 895. doi:10.1631/jzus.B1000302

Abstract

Objective: Angiogenic therapy is emerging as a potential strategy for the treatment of ischemic heart disease but is limited by a relatively short half-life of growth factors. Fibrin glue (FG) provides a reservoir for controlled- release of growth factors. The aim of this study was to evaluate the effects of basic fibroblast growth factor (bFGF) incorporating FG on angiogenesis and cardiac performance in a canine infarct model. Methods: Acute myocardial infarction was induced by ligation of the left anterior descending coronary artery (LAD). Group I (n=6) underwent ligation of LAD alone. In Group II, transmural channels were created in the infarct area (n=6). In Group III, non-transmural channels were created to locate FG cylinders containing bFGF (n=6). Eight weeks after operation, myocardial perfusion was assessed by single photon emission computed tomography, cardiac function by echocardiography, and vascular development by immunohistochemical staining. Results: Total vascular density and the number of large vessels (internal diameter ≥50 μm) were dramatically higher in Group III than in Groups I and II at eight weeks. Only the controlled-release group exhibited an improvement in regional myocardial perfusion associated with lower defect score. Animals in Group III presented improved cardiac regional systolic and diastolic functions as well as global systolic function in comparison with the other two groups. Conclusions: Enhanced and sustained angiogenic response can be achieved by controlled-release bFGF incorporating FG within transmyocardial laser channels, thus enabling improvement in myocardial perfusion and cardiac function.

Key words

AngiogenesisBasic fibroblast growth factorControlled releaseIschemic heart disease

CLC number

R54

Copyright information

© Zhejiang University and Springer-Verlag Berlin Heidelberg 2010