Journal of Zhejiang University SCIENCE B

, Volume 12, Issue 2, pp 116–125

Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection

Authors

  • Qiu-lei Lang
    • College of Life SciencesZhejiang University
    • Institute of BioengineeringZhejiang Sci-Tech University
  • Xiao-chuan Zhou
    • LC Sciences
  • Xiao-lin Zhang
    • LC Sciences
  • Rafal Drabek
    • Department of Biology and BiochemistryUniversity of Houston
  • Zhi-xiang Zuo
    • LC Sciences
  • Yong-liang Ren
    • LC Sciences
  • Tong-bin Li
    • LC Sciences
    • College of Life SciencesZhejiang University
    • Institute of BioengineeringZhejiang Sci-Tech University
    • LC Sciences
    • Department of Biology and BiochemistryUniversity of Houston
Article

DOI: 10.1631/jzus.B1000278

Cite this article as:
Lang, Q., Zhou, X., Zhang, X. et al. J. Zhejiang Univ. Sci. B (2011) 12: 116. doi:10.1631/jzus.B1000278

Abstract

A large number of plant microRNAs (miRNAs) are now documented in the miRBase, among which only 30 are for Solanum lycopersicum (tomato). Clearly, there is a far-reaching need to identify and profile the expression of miRNAs in this important crop under various physiological and pathological conditions. In this study, we used an in situ synthesized custom microarray of plant miRNAs to examine the expression and temporal presence of miRNAs in the leaves of tomato plants infected with Cucumber mosaic virus (CMV). Following computational sequence homology search and hairpin structure prediction, we identified three novel tomato miRNA precursor genes. Our results also show that, in accordance with the phenotype of the developing leaves, the tomato miRNAs are differentially expressed at different stages of plant development and that CMV infection can induce or suppress the expression of miRNAs as well as up-regulate some star miRNAs (miRNA*s) which are normally present at much lower levels. The results indicate that developmental anomalies elicited by virus infection may be caused by more complex biological processes.

Key words

Solanum lycopersicumPlant miRNACucumber mosaic virusmiRNA arraymiRNA response

CLC number

Q945.8

Supplementary material

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Copyright information

© Zhejiang University and Springer-Verlag Berlin Heidelberg 2011