Journal of Zhejiang University SCIENCE B

, Volume 11, Issue 4, pp 286–291

Accumulation of 1-deoxynojirimycin in silkworm, Bombyx mori L.

Authors

  • Hao Yin
    • Key Laboratory of Agricultural Ecology and Environment, College of ForestryShandong Agricultural University
    • Institute of Sericultural Sciences of Sichuan Province
  • Xin-qin Shi
    • Key Laboratory of Agricultural Ecology and Environment, College of ForestryShandong Agricultural University
  • Bo Sun
    • Key Laboratory of Agricultural Ecology and Environment, College of ForestryShandong Agricultural University
  • Jing-jing Ye
    • Key Laboratory of Agricultural Ecology and Environment, College of ForestryShandong Agricultural University
  • Zu-an Duan
    • Key Laboratory of Agricultural Ecology and Environment, College of ForestryShandong Agricultural University
  • Xiao-ling Zhou
    • Key Laboratory of Agricultural Ecology and Environment, College of ForestryShandong Agricultural University
    • Key Laboratory of Agricultural Ecology and Environment, College of ForestryShandong Agricultural University
    • College of Animal ScienceZhejiang University
Article

DOI: 10.1631/jzus.B0900344

Cite this article as:
Yin, H., Shi, X., Sun, B. et al. J. Zhejiang Univ. Sci. B (2010) 11: 286. doi:10.1631/jzus.B0900344

Abstract

1-deoxynojirimycin (1-DNJ) contents in the silkworm, Bombyx mori, at different developmental stages and tissues were investigated by using reverse-phase high-performance liquid chromatography. The 1-DNJ contents of silkworm larvae change significantly with their developmental stages. The male larvae showed higher accumulation efficiency of 1-DNJ than the females and also a significant variation was observed among the silkworm strains. The present results show that tissue distribution of 1-DNJ was significantly higher in blood, digestive juice, and alimentary canal, but no 1-DNJ was observed in the silkgland. Moreover, 1-DNJ was not found in silkworms fed with artificial diet that does not contain mulberry leaf powder. This proves that silkworms obtain 1-DNJ from mulberry leaves; they could not synthesize 1-DNJ by themselves. The accumulation and excretion of 1-DNJ change periodically during the larval stage. There was no 1-DNJ in the newly-hatched larvae and 1-DNJ was mainly accumulated during the early and middle stages of every instar, while excreted at later stages of larval development. Further, it is possible to extract 1-DNJ from the larval feces and it is optimal to develop the 1-DNJ related products for diabetic auxiliary therapy.

Key words

Silkworm1-deoxynojirimycin (1-DNJ)AccumulationBombyx mori

CLC number

Q966

Copyright information

© Zhejiang University and Springer-Verlag Berlin Heidelberg 2010