Article

Journal of Zhejiang University SCIENCE B

, Volume 11, Issue 4, pp 286-291

Accumulation of 1-deoxynojirimycin in silkworm, Bombyx mori L.

  • Hao YinAffiliated withKey Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural UniversityInstitute of Sericultural Sciences of Sichuan Province
  • , Xin-qin ShiAffiliated withKey Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University
  • , Bo SunAffiliated withKey Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University
  • , Jing-jing YeAffiliated withKey Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University
  • , Zu-an DuanAffiliated withKey Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University
  • , Xiao-ling ZhouAffiliated withKey Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University
  • , Wei-zheng CuiAffiliated withKey Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University Email author 
  • , Xiao-feng WuAffiliated withCollege of Animal Science, Zhejiang University Email author 

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Abstract

1-deoxynojirimycin (1-DNJ) contents in the silkworm, Bombyx mori, at different developmental stages and tissues were investigated by using reverse-phase high-performance liquid chromatography. The 1-DNJ contents of silkworm larvae change significantly with their developmental stages. The male larvae showed higher accumulation efficiency of 1-DNJ than the females and also a significant variation was observed among the silkworm strains. The present results show that tissue distribution of 1-DNJ was significantly higher in blood, digestive juice, and alimentary canal, but no 1-DNJ was observed in the silkgland. Moreover, 1-DNJ was not found in silkworms fed with artificial diet that does not contain mulberry leaf powder. This proves that silkworms obtain 1-DNJ from mulberry leaves; they could not synthesize 1-DNJ by themselves. The accumulation and excretion of 1-DNJ change periodically during the larval stage. There was no 1-DNJ in the newly-hatched larvae and 1-DNJ was mainly accumulated during the early and middle stages of every instar, while excreted at later stages of larval development. Further, it is possible to extract 1-DNJ from the larval feces and it is optimal to develop the 1-DNJ related products for diabetic auxiliary therapy.

Key words

Silkworm 1-deoxynojirimycin (1-DNJ) Accumulation Bombyx mori

CLC number

Q966