Hypothetical mode of action of earthworm extract with hepatoprotective and antioxidant properties

  • Mariappan Balamurugan
  • Kasi Parthasarathi
  • Lalpet Souri Ranganathan
  • Edwin L. Cooper
Article

DOI: 10.1631/jzus.B0720194

Cite this article as:
Balamurugan, M., Parthasarathi, K., Ranganathan, L.S. et al. J. Zhejiang Univ. Sci. B (2008) 9: 141. doi:10.1631/jzus.B0720194

Abstract

The hepatoprotective potential of earthworm extract (EE) (Lampito mauritii, Kinberg) was evaluated against paracetamol-induced liver injury in Wistar albino rat, in comparison with silymarin, the standard hepatoprotective drug. We observed a reduction in liver antioxidants, such as glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) and in serum total protein, and an increase in serum alkaline phosphatase (ALP), serum aspertate aminotranferase (AST), serum alanine aminotranferase (ALT), bilirubin and liver thiobarbituric acid reactive substances (TBARS) due to liver injury in the paracetamol-administered rats (2 g/kg). On the contrary, increased activities of liver GSH, SOD, GPx, CAT and serum total protein level, and decrease in the contents of serum ALP, AST, ALT, bilirubin and liver TBARS were observed in rats administered with different doses of EE (100, 200 and 300 mg/kg), which are similar to the activities of hepato-protective drug silymarin (150 mg/kg). The mode of action of EE as evidenced by the above parameters may suggest that EE, on the one hand, prevents the formation of the reactive oxygen groups, or scavenges these groups, thereby preventing the damage on the hepatic cells, and, on the other hand, modulates the genes responsible for synthesis of antioxidant enzymes such as GPx, CAT and SOD in liver tissue and decreases the serum enzymatic activities such as ALP, AST and ALT.

Key words

Earthworm extract Hepatoprotective potential Antioxidant Paracetamol Lipid peroxidation 

Document code

CLC number

Q95 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Mariappan Balamurugan
    • 1
  • Kasi Parthasarathi
    • 1
  • Lalpet Souri Ranganathan
    • 2
  • Edwin L. Cooper
    • 3
  1. 1.Division of Vermibiotechnology, Department of ZoologyAnnamalai UniversityNagarIndia
  2. 2.VLB Janakiammal College of Arts and ScienceCoimbatoreIndia
  3. 3.Laboratory of Comparative Immunology, Department of NeurobiologyUCLA Medical Centre, University of California Los AngelesUSA

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