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Physicochemical aspects of Trichosporon cutaneum CCY 30-5-10 adhesion and biofilm formation potential on cellophane

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Abstract

This paper focuses on the adhesion and biofilm formation potential of cellulolytic yeast Trichosporon cutaneum CCY 30-5-10 on solid cellophane from a novel perspective. First, physicochemical characterisation of the cells and carrier (cellophane) was performed to evaluate the effect of different culture media (complex vs mineral) on yeast cell adhesion. (Un)favourable adhesion conditions were predicted using the thermodynamic approach. Next, the ability of the cells to colonise the carrier under the above conditions was quantified and the biofilm structure was characterised using image analysis. The approaches described were found suitable to predict and experimentally verify favourable (cell-solid) adhesion, i.e. the hydrophobic and low electron-donor nature of cellophane together with hydrophobic cells (obtained when cultivated in a complex culture medium) were found to have a major impact in defining successful yeast adhesion with subsequent biofilm formation.

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Authors and Affiliations

  1. Institute of Chemical Technology Prague, Technická 5, 166 28, Prague 6, Czech Republic

    Jitka Dostálková, Gita Procházková, Vladimír Jirků, Miloslav Suchánek & Tomáš Brányik

  2. Technical University of Liberec, Studentská 2, 461 17, Liberec, Czech Republic

    Lucie Křiklavová & Tomáš Lederer

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  1. Jitka Dostálková
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  2. Gita Procházková
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  3. Vladimír Jirků
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  4. Lucie Křiklavová
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Correspondence to Tomáš Brányik.

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Dostálková, J., Procházková, G., Jirků, V. et al. Physicochemical aspects of Trichosporon cutaneum CCY 30-5-10 adhesion and biofilm formation potential on cellophane. Chem. Pap. 69, 425–432 (2015). https://doi.org/10.1515/chempap-2015-0046

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