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Immobilisation of Aspergillus oryzae α-amylase and Aspergillus niger glucoamylase enzymes as cross-linked enzyme aggregates

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Abstract

Cross-linked enzyme aggregates (CLEA) of Aspergillus oryzea α-amylase (AoAA) and Aspergillus niger glucoamylase (AnGA) were prepared using glutaraldehyde and dextran polyaldehyde as cross-linkers. The maximum activity recoveries for glutaraldehyde cross-linking were 21.8 % and 41.2 %, respectively. The addition of a proteic feeder (bovine serum albumin) exhibited a negative effect on the activity recoveries for both enzymes. Dextran polyaldehyde was used as a cross-linking agent instead of glutaraldehyde to reduce the activity losses. As a result, an activity recovery of 60.0 % was obtained for Aspergillus oryzea α-amylase. On the other hand, no activity recovery was observed for Aspergillus niger glucoamylase due to the latter enzyme’s affinity for dextran.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Sciences and Arts, Canakkale Onsekiz Mart University, 17200, Canakkale, Turkey

    A. Sercan Sahutoglu & Cahit Akgul

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  1. A. Sercan Sahutoglu
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  2. Cahit Akgul
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Correspondence to A. Sercan Sahutoglu.

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Sahutoglu, A.S., Akgul, C. Immobilisation of Aspergillus oryzae α-amylase and Aspergillus niger glucoamylase enzymes as cross-linked enzyme aggregates. Chem. Pap. 69, 433–439 (2015). https://doi.org/10.1515/chempap-2015-0031

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