Laminin-5 γ2-chain expression and DNA ploidy as predictors of prognosis in endometrial carcinoma
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Expression of the laminin-5 γ2-chain in carcinoma cells has been implicated in tumor invasion. The aim was to investigate the expression and prognostic significance of the In-5 γ2-chain compared with clinicopathological factors and tumor cell DNA ploidy in endometrial carcinoma. Histological specimens from 80 endometrial carcinomas were examined with respect to immunohistochemical In-5 γ2-chain expression and correlated to the clinicopathological characteristics, DNA ploidy, and survival. Sixty-eight of 80 investigated cases were judged to be positive for the In-5 γ2-chain. Ln-5 γ2-chain did not show any correlation to stage, histopathological subtype, grade, and DNA ploidy. In univariate analyses, advanced stage (p<0.001), nonendometrioid carcinoma (p=0.030), low grade (p<0.001), aneuploid tumors (p<0.001), and In-5 γ2-chain expression (p=0.017) were highly associated with poor survival. Aneuploid tumors in combination with strong In-5 γ2-chain expression were significant predictors (p<0.001) of poor prognosis. In multivariate analyses including stage, histopathological subgroup, grade, DNA ploidy, and In-5 γ2-chain expression, all lost their significant prognostic information except for stage (p<0.001) and grade (p<0.05). Ln-5 γ2-chain expression and DNA ploidy both as a single parameter and in combination were demonstrated to be significant prognostic factors in univariate analysis. However, stage and grade provided more useful clinical information beyond histopathological subgroup, DNA ploidy, and In-5 γ2-chain expression. The results also indicate that In-5 γ2-chain expression is upregulated during the progression of endometrial carcinoma.
- Pettersson, F. (ed.) (1994). Annual Report on the Results of Treatment in Gynecological Cancer, 1987–93. FIGO, Stockholm, Vol. 22.
- Geisler, J.P., Wiemann, M.C., Zhou, Z., Miller, G.A. and Geisler, H.E. (1996). p53 as a prognostic indicator in endometrial cancer. Gynecol. Oncol. 61(2):245–248. CrossRef
- Strang, P., Nordstom, B., Nilsson, S., Bergstrom, R. and Tribukait, B. (1996). Mutant p53 protein as a predictor of survival in endometrial carcinoma. Eur. J. Cancer 32A(4):598–602. CrossRef
- Salvesen, H.B., Iversen, O.E. and Akslen, L.A. (1999). Prognostic significance of angiogenesis and Ki-67, p53, and p21 expression: a population-based endometrial carcinoma study. J. Clin. Oncol. 17(5):1382–1390.
- Geisler, J.P., et al. (1999). MIB-1 in endometrial carcinoma: prognostic significance with 5-year follow-up. Gynecol. Oncol. 75(3):432–436. CrossRef
- Nordstrom, B., Strang, P., Bergstrom, R., Nilsson, S. and Tribukait, B. (1996). A comparison of proliferation markers and their prognostic value for women with endometrial carcinoma. Ki-67, proliferating cell nuclear antigen, and flow cytometric S-phase fraction. Cancer 78(9):1942–1951. CrossRef
- Stendahl, U., Wagenius, G., Strang, P. and Tribukait, B. (1988). Flow cytometry in invasive endometrial carcinoma. Correlations between DNA content S-phase rate and clinical parameters. In Vivo 2(2):123–127.
- Lundgren, C., et al. (2002). Nuclear DNA content, proliferative activity, and p53 expression related to clinical and histopathologic features in endometrial carcinoma. Int. J. Gynecol. Cancer 12(1):110–118. CrossRef
- Biesterfeld, S., Leitloff, M., Rath, W. and Schroder, W. (2001). DNA image cytometry in the differential diagnosis of endometrial hyperplasia and adenocarcinoma. Anal. Quant. Cytol. Histol. 23(2):123–128.
- Pyke, C., et al. (1994). The gamma 2 chain of kalinin/laminin 5 is preferentially expressed in invading malignant cells in human cancers. Am. J. Pathol. 145(4):782–791.
- Salo, S., et al. (1999). Laminin-5 promotes adhesion and migration of epithelial cells: identification of a migration-related element in the gamma2 chain gene (LAMC2) with activity in transgenic mice. Matrix Biol. 18(2):197–210. CrossRef
- Giannelli, G. and Antonaci, S. (2000). Biological and clinical relevance of laminin-5 in cancer. Clin. Exp. Metastasis 18(6):439–443. CrossRef
- Gagnoux-Palacios, L., et al. (2001). The short arm of the laminin gamma2 chain plays a pivotal role in the incorporation of laminin 5 into the extracellular matrix and in cell adhesion. J. Cell Biol. 153(4):835–850. CrossRef
- Pyke, C., et al. (1995). Laminin-5 is a marker of invading cancer cells in some human carcinomas and is coexpressed with the receptor for urokinase plasminogen activator in budding cancer cells in colon adenocarcinomas. Cancer Res. 55(18):4132–4139.
- Sordat, I., et al. (1998). Differential expression of laminin-5 subunits and integrin receptors in human colorectal neoplasia. J. Pathol. 185(1):44–52. CrossRef
- Lenander, C., et al. (2001). Laminin-5 gamma 2 chain expression correlates with unfavorable prognosis in colon carcinomas. Anal. Cell Pathol. 22(4):201–209.
- Kainulainen, T., et al. (1997). Altered distribution and synthesis of laminin-5 (kalinin) in oral lichen planus, epithelial dysplasias and squamous cell carcinomas. Br. J. Dermatol. 136(3):331–336. CrossRef
- Skyldberg, B., et al. (1999). Laminin-5 as a marker of invasiveness in cervical lesions. J. Natl. Cancer Inst. 91(21):1882–1887. CrossRef
- Fukushima, N., Sakamoto, M. and Hirohashi, S. (2001). Expression of laminin-5-gamma-2 chain in intraductal papillary-mucinous and invasive ductal tumors of the pancreas. Mod. Pathol. 14(5):404–409. CrossRef
- Hellman, K., et al. (2000). Cancer of the vagina: laminin-5 gamma2 chain expression and prognosis. Int. J. Gynecol. Cancer 10(5):391–396. CrossRef
- Yamamoto, H., Itoh, F., Iku, S., Hosokawa, M. and Imai, K. (2001). Expression of the gamma(2) chain of laminin-5 at the invasive front is associated with recurrence and poor prognosis in human esophageal squamous cell carcinoma. Clin. Cancer Res. 7(4):896–900.
- Nordstrom, B., et al. (2002). Laminin-5 gamma 2 chain as an invasivity marker for uni- and multifocal lesions in the lower anogenital tract. Int. J. Gynecol. Cancer 12(1):105–109. CrossRef
- Nordemar, S., et al. (2001). Laminin-5 as a predictor of invasiveness in cancer in situ lesions of the larynx. Anticancer Res. 21(1B):509–512.
- Hao, J., et al. (2001). Investigation into the mechanism of the loss of laminin 5 (alpha3beta3gamma2) expression in prostate cancer. Am. J. Pathol. 158(3):1129–1135.
- Martin, K.J., et al. (1998). Down-regulation of laminin-5 in breast carcinoma cells. Mol. Med. 4(9):602–613.
- Sobin, L. (ed.) (1978). A Coded Compendium of Internanational Histological Classifications of Tumours, 11th ed. World Health Organization, Geneva.
- FIGO (1989). FIGO stages–1988 revisions: Vulva, ovary, corpus. Gynecol. Oncol. 35:125–127. CrossRef
- Gaub, J.A. and Zetterberg, A. (1965). Quantitative cytochemical aspects of a combined Feulgen naphtol-yellow-s staining procedure for the simultaneous determination of nuclear and cytoplasmic proteins and DNA in mammalian cells. Exp. Cell Res. 92:323–332. CrossRef
- Steinbeck, R.G., Auer, G.U. and Zetterberg, A.D. (1999). Reliability and significance of DNA measurements in interphase nuclei and division figures in histological sections. Eur. J. Cancer 35(5):787–795. CrossRef
- Auer, G. and Tribukait, B. (1980). Comparative single cell and flow DNA analysis in aspiration biopsies from breast carcinomas. Acta Pathol. Microbiol. Scand. [A] 88(6):355–358.
- Hellstrom, A., Hue, J., Silfverswärd, C. and Auer, G. (1994). DNA-ploidy and mutant p53 overexpression in primary fallopian tube cancer. Int. J. Gynecol. Cancer 4:408–413. CrossRef
- Auer, G.U., Caspersson, T.O. and Wallgren, A.S. (1980). DNA content and survival in mammary carcinoma. Anal. Quant. Cytol. 2(3):161–165.
- Nordstrom, B., Strang, P., Lindgren, A., Bergstrom, R. and Tribukait, B. (1996). Carcinoma of the endometrium: do the nuclear grade and DNA ploidy provide more prognostic information than do the FIGO and WHO classifications? Int. J. Gynecol. Pathol. 15(3):191–201. CrossRef
- Zaino, R.J., Davis, A.T., Ohlsson-Wilhelm, B.M. and Brunetto, V.L. (1998). DNA content is an independent prognostic indicator in endometrial adenocarcinoma. A Gynecologic Oncology Group study. Int. J. Gynecol. Pathol. 17(4):312–319. CrossRef
- Laminin-5 γ2-chain expression and DNA ploidy as predictors of prognosis in endometrial carcinoma
Volume 20, Issue 2 , pp 147-155
- Cover Date
- Print ISSN
- Online ISSN
- Humana Press
- Additional Links
- Endometrial carcinoma
- laminin-5 γ2 chain
- DNA ploidy
- basement membranes
- Industry Sectors
- Author Affiliations
- 1. Department of Gynaecologic Oncology, Radiumhemmet, Karolinska Hospital, S 171-76, Stockholm, Sweden
- 2. Department of Pathology and Cell Analysis, Medical Biochemistry and Biophysics, Karolinska Hospital and Institute, S 171-76, Stockholm, Sweden
- 3. Department of Cancer Epidemiology, Medical Biochemistry and Biophysics, Karolinska Hospital and Institute, S 171-76, Stockholm, Sweden
- 4. Department of Matrix Biology, Medical Biochemistry and Biophysics, Karolinska Hospital and Institute, S 171-76, Stockholm, Sweden