Molecular Biotechnology

, Volume 20, Issue 1, pp 85-93

First online:

Aqueous two-phase extraction and purification of animal proteins

  • Michael J. BolandAffiliated withNew Zealand Dairy Research Institute Email author 

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Aqueous two-phase systems provide a rapid, easily scalable method for separation of soluble proteins from insoluble materials and other undesired proteins. The method can be operated in continuous mode. It is particularly useful for animal proteins, as it overcomes difficulties of other methods in removing bulk insoluble material, while at the same time providing purification with respect to total soluble protein. This article describes the development of methods for aqueous two-phase extraction and purification of animal proteins, at both laboratory and pilot scale. The strengths, weaknesses, and possible future prospects for the method are discussed.

Index Entries

“Aqueous two-phase” protein enzyme animal purification separation