Abstract
Carbon nanotubes are strong, flexible, conduct electrical current, and can be functionalized with different molecules, properties that may be useful in basic and applied neuroscience research. We report the first application of carbon nanotube technology to neuroscience research. Methods were developed for growing embryonic rat-brain neurons on multiwalled carbon nanotubes. On unmodified nanotubes, neurons extend only one or two neurites, which exhibit very few branches. In contrast, neurons grown on nanotubes coated with the bioactive molecule 4-hydroxynonenal elaborate multiple neurites, which exhibit extensive branching. These findings establish the feasability of using nanotubes as substrates for nerve cell growth and as probes of neuronal function at the nanometer scale.
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Andrews R., Jacques D., Rao A. M., Derbyshire F., Qian D., Fan X., et al. (1999) Continuous production of aligned carbon nanotubes: a step closer to commercial realization. Chem. Phys. Lett. 303, 467.
Carini R., Bellomo G., Paradisi L., Dianzani M. U., and Albano E. (1996) 4-Hydroxynonental triggers Ca2+ influx in isolated rat hepatocytes. Biochem. Biophys. Res. Commun. 18, 772–776.
Chen J., Hamon M. A., Hu H., Chen Y., Rao A. M., Eklund P. C., and Haddon R. C. (1998) Solution properties of single-walled carbon nanotubes. Science 282, 95–98.
Esterbauer H., Schaur R. J., and Zollner H. (1991) Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Rad. Biol. Med. 11, 81–128.
Fan S., Chapline M. G., Franklin N. R., Tombler T. W., Cassell A. M., and Dai H. (1999) Self-oriented regular arrays of carbon nanotubes and their field emission properties. Science 283, 512–514.
Goodman C. S. (1996) Mechanisms and molecules that control growth cone guidance. Annu. Rev. Neurosci. 19, 341–377.
Hamon M. A., Chen J., Hu H., Chen Y., Rao A. M., Eklund P. C., and Haddon R. C. (1999) Dissolution of single-walled carbon nanotubes. Adv. Mater. 11, 834–840.
Journet C., Maser W. K., Bernier P., Loiseau A., Lamy de la Chapelle M., Lefrant S., et al. (1997) Large scale production of single wall carbon nanotubes by the electric arc technique. Nature 388, 756–758.
Kater S. B., Mattson M. P., Cohan C., and Connor J. (1988) Calcium regulation of the neuronal growth cone. Trends Neurosci. 11, 315–321.
Lustgarten J. H., Proctor M., Haroun R. I., Avellino A. M., Pindzola A. A., and Kliot M. (1991) Semipermeable polymer tubes provide a microenvironment for in vivo analysis of dorsal root regeneration. J. Biomech. Eng. 113, 184–188.
Mark R. J., Lovell M. A., Markesbery W. R., Uchida K., and Mattson M. P. (1997) A role for 4-hydroxynonenal in disruption of ion homeostasis and neuronal death induced by amyloid β-peptide. J. Neurochem. 68, 255–264.
Mattson M. P. (1988) Neurotransmitters in the regulation of neuronal cytoarchitecture. Brain Res. Rev. 13, 179–212.
Mattson M. P. and Kater S. B. (1987) Calcium regulation of neurite elongation and growth cone motility. J. Neurosci. 7, 4034–4043.
Mattson M. P. and Kater S. B. (1988) Intracellular messengers in the generation and degeneration of hippo-campal neuroarchitecture. J. Neurosci. Res. 21, 447–464.
Mattson M. P., Dou P., and Kater S. B. (1988) Out-growth-regulating actions of glutamate in isolated hippo-campal pyramidal neurons. J. Neurosci. 8, 2087–2100.
Mattson M. P., Fu W., Waeg G., and Uchida K. (1997) 4-hydroxynonenal, a product of lipid peroxidation, inhibits dephosphorylation of the microtubule-associated protein tau. NeuroReport 8, 2275–2281.
Mattson M. P. and Partin J. (1999) Evidence for mitochondrial control of neuronal polarity. J. Neurosci. Res. 56, 8–20.
Rao A. M., Richter E., Bandow S., Chase B., Eklund P. C., Williams K. A., et al. (1997) Diameter-selective Raman scattering from vibrational modes in carbon nanotubes. Science 275, 187–191.
Ren Z. F., Huang Z. P., Xu J. W., Wang J. H., Bush P., Siegal M. P., and Provencio P. N. (1998) Synthesis of large arrays of well-aligned carbon nanotubes on glass. Science 282, 1105–1107.
Song H. J. and Poo M. M. (1999) Signal transduction underlying growth cone guidance by diffusible factors. Curr. Opin. Neurobiol. 9, 355–363.
Suter D. M. and Forscher P. (1998) An emerging link between cytoskeletal dynamics and cell adhesion molecules in growth cone guidance. Curr. Opin. Neurobiol. 8, 106–116.
Tans S. J., Vershueren A. R. M., and Dekker C. (1998) Room temperature transistor based on a single carbon nanotube. Nature 393, 49–52.
Thess A., Lee R., Nikolaev P., Dai H., Petit P., Robert J., et al. (1996) Crystalline ropes of metallic carbon nanotubes. Science 273, 483–487.
Uchida K. and Stadtman E. R. (1992) Modification of histidine residues in proteins by reaction with 4-hydroxynonenal. Proc. Natl. Acad. Sci. USA 89, 4544–4548.
Waeg G., Dimsity G., and Esterbauer H. (1996) Monoclonal antibodies for detection of 4-hydroxynonenal modified proteins. Free Rad. Res. 25, 149–159.
Wong E. W., Sheehan P. E., and Lieber C. M. (1997) Nanobeam mechanics: elasticity, strength, and toughness of nanorods and nanotubes. Science 277, 1971–1975.
Wong S. S., Joselevich E., Woolley A. T., Caung C. L., and Lieber C. M. (1998) Covalently functionalized nanotubes as nanometre-sized probes in chemistry and biology. Nature 394, 52–55.
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Mattson, M.P., Haddon, R.C. & Rao, A.M. Molecular functionalization of carbon nanotubes and use as substrates for neuronal growth. J Mol Neurosci 14, 175–182 (2000). https://doi.org/10.1385/JMN:14:3:175
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DOI: https://doi.org/10.1385/JMN:14:3:175