Abstract
We investigated the influence of temperature, pressure, exposure time, and decompression rate on lipase activity in high-pressure CO2 medium. A high-pressure, variable-volume view cell was employed in the experiments, varying the temperature from 30 to 70°C in the pressure range of 70–250 bar at various high-pressure exposure times (60–360 min) and adopting several decompression rates (10–200 kg/[m3·min]). The results obtained show that an increase in temperature and density led to an enhancement of enzyme activity losses while the decompression rates had a weak influence on enzyme inactivation.
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Lanza, M., Priamo, W.L., Oliveira, J.V. et al. The effect of temperature, pressure, exposure time, and depressurization rate on lipase activity in SCCO2 . Appl Biochem Biotechnol 113, 181–187 (2004). https://doi.org/10.1385/ABAB:113:1-3:181
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DOI: https://doi.org/10.1385/ABAB:113:1-3:181