Optimization of SO2-catalyzed steam pretreatment of corn fiber for ethanol production
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
A batch reactor was employed to steam explode corn fiber at various degrees of severity to evaluate the potential of using this feedstock as part of an enzymatically mediated cellulose-to-ethanol process. Severity was controlled by altering temperature (150–230°C), residence time (1–9 min), and SO2 concentration (0–6% [w/w] dry matter). The effects of varying the different parameters were assessed by response surface modeling. The results indicated that maximum sugar yields (hemicellulose-derived water soluble, and cellulose-derived following enzymatic hydrolysis) were recovered from corn fiber pretreated at 190°C for 5 minutes after exposure to 3% SO2. Sequential SO2-catalyzed steam explosion and enzymatic hydrolysis resulted in a conversion efficiency of 81% of the combined original hemicellulose and cellulose in the corn fiber to monomeric sugars. An additional posthydrolysis step performed on water soluble hemicellulose stream increased the concentration of sugars available for fermentation by 10%, resulting in the high conversion efficiency of 91%. Saccharomyces cerevisiae was able to ferment the resultant corn fiber hydrolysates, perhydrolysate, and liquid fraction from the posthydrolysis steps to 89, 94, and 85% of theoretical ethanol conversion, respectively. It was apparent that all of the parameters investigated during the steam explosion pretreatment had a significant effect on sugar recovery, inhibitory formation, enzymatic conversion efficiency, and fermentation capacity of the yeast.
- Anderson, R. A. and Watson, S. A. (1982), in Handbook of Processing and Utilization in Agriculture, vol. 2, Wolff, I. A. ed., CRC, Boca Raton, FL, pp. 31–61.
- Grohmann, K. and Bothast, R. J. (1996), Process Biochem. 32, 405–415. CrossRef
- Moniruzzaman, M., Dale, B. E., Hespell, R. B., and Bothast, R. J. (1997), Appl. Biochem. Biotechnol. 67, 113–126. CrossRef
- Allen, S. G., Schulman D., Lichwa, J., and Antal, M. J. (2001), Ind. Eng. Chem. Res. 40, 2934–2941. CrossRef
- Bura, R., Mansfield S. D., Saddler J. N., and Bothast R. J (2002), Appl. Biochem. Biotechnol. 98–100, 59–72. CrossRef
- Overend, R. P. and Chornet, E. (1987), Phil. Trans. R. Soc. Lond. 321, 523–536.
- TAPPI, Technical Association of the Pulp and Paper Industry (1998), TAPPI Standard Methods, T-222 om-98, TAPPI Press, Atlanta, GA.
- Ghose, T. K. (1987), Pure Appl. Chem. 59, 257–268.
- Box, G. E. P., Hunter, W. G., and Hunter J. S. (1978), in Statistics for Experimenters, John Wiley & Sons, New York, NY, pp. 510–539.
- Clark, T. A. and Mackie, K. L. (1987), J. Wood Chem. Technol. 7, 373–403.
- Boussaid, A., Jarvis J., Gregg, D. J., and Saddler, J. N. (1997), in The Third Biomass Conference of the Americas, Overend, R. P. and Chornet, E., eds., Montreal, Canada, Elsevier Science, pp.878–880.
- Stenberg, K., Tengborg, Ch., Galbe, M., and Zacchi, G. (1998), J. Chem. Technol. Biotechnol. 71, 299–308. CrossRef
- San Martin, R., Perez, C., and Briones, R. (1995), Bioresour. Technol. 53, 217–223. CrossRef
- Shevchenko, S. M., Chang, K., Robinson, J., and Saddler, J. N. (2000), Bioresour. Biotechnol. 72, 207–211. CrossRef
- Excoffier, G., Toussaint, B., and Vignon, M. R. (1991), Biotechnol. Bioeng. 38, 1308–1317. CrossRef
- Hespell, R. B., O’Bryan, P. J., Moniruzzaman, M., and Bothast R. J. (1997), Appl. Biochem. Biotechnol. 62, 87–97.
- Montgomery, R. and Smith, J. (1970), J. Am. Chem. Soc. 79, 695–699. CrossRef
- Delgenes, J. P., Moletta, R., and Navarro J. M. (1996), Enzyme Microb. Technol. 19, 220–225. CrossRef
- Clark, T. A., Mackie, K. L., Dare, P., H. and McDonald, A. G. (1989), J. Wood Chem. Technol. 9, 135–166.
- Schwald, W., Smaridge, T., Chan, M., Breuil, C., and Saddler, J. N. (1987), in Enzyme Systems for Lignocellulose Degradation, Coughlan, M. P. ed., Elsevier, New York, NY, pp. 231–242.
- Optimization of SO2-catalyzed steam pretreatment of corn fiber for ethanol production
Applied Biochemistry and Biotechnology
Volume 106, Issue 1-3 , pp 319-335
- Cover Date
- Print ISSN
- Online ISSN
- Humana Press
- Additional Links
- Corn fiber
- steam pretreatment
- enzymatic hydrolysis
- Industry Sectors
- Author Affiliations
- 1. Forest Products Biotechnology, Department of Wood Science, University of British Columbia, 2424 Main Mall, V6T 1Z4, Vancouver, BC, Canada
- 2. Fermentation Biochemistry, National Center for Agricultural Utilization Research, USDA, ARS, 1815 N. University Street, 61604, Peoria, IL