Molecular Biotechnology

, Volume 31, Issue 2, pp 129–135

A simple and efficient method for isolation of DNA in high mucilaginous plant tissues

  • Ileana Echevarría-Machado
  • Lucila A. Sánchez-Cach
  • Cecilia Hernández-Zepeda
  • Renata Rivera-Madrid
  • Oscar A. Moreno-Valenzuela
Protocols

DOI: 10.1385/MB:31:2:129

Cite this article as:
Echevarría-Machado, I., Sánchez-Cach, L.A., Hernández-Zepeda, C. et al. Mol Biotechnol (2005) 31: 129. doi:10.1385/MB:31:2:129

Abstract

A protocol is described for rapid DNA isolation from Malvaceae plant species and different tissues of Bixaceae that contain large amounts of polysaccharides, polyphenols, and pigments that interfere with DNA extractions. The method is a modification of Dellaporta et al. The current protocol is simple, and no phenolchloroform extraction, ethanol, or isopropranol precipitation is required. The method is based in the incubation of soluble DNA with silica, mix in batch during the extraction. The procedure can be completed in 2 h and many samples can be processed at the same time. DNA of excellent quality was recovered and used for polymerase chain reaction (PCR) amplification, restriction enzyme digestion, and Southern blot analysis. The method was used with healthy Bixa orellana and virus-infected Malvaceae plants.

Index Entries

DNA extraction Malvaceae PCR Bixa orellana 

Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Ileana Echevarría-Machado
  • Lucila A. Sánchez-Cach
  • Cecilia Hernández-Zepeda
  • Renata Rivera-Madrid
  • Oscar A. Moreno-Valenzuela
    • 1
  1. 1.Unidad de Bioquímica y Biología Molecular de PlantasCentro de Investigación Científica de YucatánMérida, YucatánMéxico

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