Molecular Biotechnology

, Volume 26, Issue 2, pp 147-163

First online:

MALDI-TOF mass spectrometry

A versatile tool for high-performance DNA analysis
  • Christian JurinkeAffiliated withSequenom, Inc., Johns Hopkins Court Email author 
  • , Paul Oeth
  • , Dirk van den Boom

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has developed during the past decade into a versatile tool for biopolymer analysis. The aim of this review is to summarize this development and outline the applications, which have been enabled for routine use in the field of nucleic acid analysis. These include the anlaysis of mutations, the resequencing of amplicons with a known reference sequence, and the quantitative analysis of gene expression and allelic frequencies in complex DNA mixtures.

Index Entries

MALDI-TOF SNP analysis genotyping quantitative MALDI-TOF gene-expression analysis resequencing SNP discovery DNA pooling