Molecular Biotechnology

, Volume 25, Issue 1, pp 53–61

Mimotopes of tumor-associated T-cell epitopes for cancer vaccines determined with combinatorial peptide libraries

Authors

    • Department of Dermatology and Allergy, Medical Faculty CharitéHumboldt University
  • Karl-Heinz Wiesmüller
    • EMC Microcollections GmbH and Institute of Organic Chemistry
  • Peter Walden
    • Department of Dermatology and Allergy, Medical Faculty CharitéHumboldt University
Protocol

DOI: 10.1385/MB:25:1:53

Cite this article as:
Sherev, T., Wiesmüller, K. & Walden, P. Mol Biotechnol (2003) 25: 53. doi:10.1385/MB:25:1:53

Abstract

Cytotoxic T-cells are the most important effector cells in immune responses against tumors. The identification of tumor-associated epitopes for these cells, therefore, has become a key aspect of the development of cancer vaccines. Here, we describe a new approach to the determination of tumor-associated T-cell epitopes which employs combinatorial peptide libraries with singly defined sequence positions in a randomized context. The analysis of the responses of a T-cell clone to these libraries yields the amino acid constituents of the epitope which can be combined to obtain mimotopes that are suitable as vaccine antigens for the induction of tumor-specific responses.

Index Entries

Brefeldin-Acombinatorial peptide librarySEREX, serological analysis of tumor antigens by recombinant cDNA expression cloningTAA, tumor-associated antigenTATE, tumor-associated T-cell epitope

Copyright information

© Humana Press Inc 2003