Mimotopes of tumor-associated T-cell epitopes for cancer vaccines determined with combinatorial peptide libraries
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Cytotoxic T-cells are the most important effector cells in immune responses against tumors. The identification of tumor-associated epitopes for these cells, therefore, has become a key aspect of the development of cancer vaccines. Here, we describe a new approach to the determination of tumor-associated T-cell epitopes which employs combinatorial peptide libraries with singly defined sequence positions in a randomized context. The analysis of the responses of a T-cell clone to these libraries yields the amino acid constituents of the epitope which can be combined to obtain mimotopes that are suitable as vaccine antigens for the induction of tumor-specific responses.
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- Mimotopes of tumor-associated T-cell epitopes for cancer vaccines determined with combinatorial peptide libraries
Volume 25, Issue 1 , pp 53-61
- Cover Date
- Print ISSN
- Online ISSN
- Humana Press
- Additional Links
- combinatorial peptide library
- SEREX, serological analysis of tumor antigens by recombinant cDNA expression cloning
- TAA, tumor-associated antigen
- TATE, tumor-associated T-cell epitope
- Industry Sectors
- Author Affiliations
- 1. Department of Dermatology and Allergy, Medical Faculty Charité, Humboldt University, Schumannstrasse 20/21, 10117, Berlin, Germany
- 2. EMC Microcollections GmbH and Institute of Organic Chemistry, 72074, Tübingen, Germany