Magnetite-alginate beads for purification of some starch degrading enzymes
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- Teotia, S. & Gupta, M.N. Mol Biotechnol (2002) 20: 231. doi:10.1385/MB:20:3:231
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Starch degrading enzymes, viz., β-amylase, glucoamylase, and pullulanase, were purified using magnetite-alginate beads. In each case, the enzyme activity was eluted by using 1.0 M maltose. β-Amylase (sweet potato), glucoamylase (Aspergillus niger), and pullulanase (Bacillus acidopullulyticus) from their crude preparations were purified 37-, 31-, and 49-fold with 86, 87, and 95% activity recovery, respectively.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed single band in each case.