Research

Molecular Biotechnology

, Volume 20, Issue 3, pp 231-237

First online:

Magnetite-alginate beads for purification of some starch degrading enzymes

  • Sunita Teotia
  • , M. N. GuptaAffiliated withChemistry Department, Indian Institute of Technology, Hauz Khas Email author 

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Abstract

Starch degrading enzymes, viz., β-amylase, glucoamylase, and pullulanase, were purified using magnetite-alginate beads. In each case, the enzyme activity was eluted by using 1.0 M maltose. β-Amylase (sweet potato), glucoamylase (Aspergillus niger), and pullulanase (Bacillus acidopullulyticus) from their crude preparations were purified 37-, 31-, and 49-fold with 86, 87, and 95% activity recovery, respectively.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed single band in each case.

Index Entries

Affinity separation β-amylase glucoamylase pullulanase magnetite-alginate beads Aspergillus niger Bacillus acidopullulyticus