“De novo” sequencing of peptides recovered from in-gel digested proteins by nanoelectrospray tandem mass spectrometry
- Cite this article as:
- Shevchenko, A., Chernushevich, I., Shevchenko, A. et al. Mol Biotechnol (2002) 20: 107. doi:10.1385/MB:20:1:107
- 210 Views
Proteins separated by one-dimensional or two-dimensional gel electrophoresis can be digested in-gel with trypsin and the recovered peptides can be sequenced de novo using triple quadrupole or hybrid quadrupole time-of-flight instruments equipped with a nanoelectrospray ion source. The peptide sequences determined provide useful information for identification of proteins by homology searching for cloning of the cognate genes by PCR based approaches.