, Volume 20, Issue 1, pp 107-118

“De novo” sequencing of peptides recovered from in-gel digested proteins by nanoelectrospray tandem mass spectrometry

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Abstract

Proteins separated by one-dimensional or two-dimensional gel electrophoresis can be digested in-gel with trypsin and the recovered peptides can be sequenced de novo using triple quadrupole or hybrid quadrupole time-of-flight instruments equipped with a nanoelectrospray ion source. The peptide sequences determined provide useful information for identification of proteins by homology searching for cloning of the cognate genes by PCR based approaches.