Molecular Biotechnology

, Volume 17, Issue 3, pp 225–238

Scale-up of suspension and anchorage-dependent animal cells

Protocol

DOI: 10.1385/MB:17:3:225

Cite this article as:
Griffiths, B. Mol Biotechnol (2001) 17: 225. doi:10.1385/MB:17:3:225

Abstract

Alternative culture processes for laboratory scale-up (to 20 L) are described for both suspension and anchorage-dependent cells. Systems range from simple multiple culture units such as the roller bottle, through stirred suspension and microcarrier unit bioreactors, to highly sophisticated perfusion culture capable of maintaining cells at densities of about 108/mL. Critical parameters in scale-up are discussed, and the advantages and disadvantages of each culture system are critically evaluated.

Index Entries

Mammalian cell culturesuspension culturemicrocarrier cultureculture scale-upanchorage-dependent cellsanimal cell bioreactors

Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  1. 1.SC&PPorton, SalisburyUK