Nuclear immunostaining in rat neuronal cells using two anti-kir2.2 ion channel polyclonal antibodies
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The inwardly rectifying potassium ion channel Kir2.2 has recently been demonstrated to have nuclear and plasma membrane subcellular localization. Nuclear expression of Kir2.2 is controversial, as a functional role for Kir2.0 potassium channels in the nucleus has not been investigated. However, in this report we have demonstrated Kir2.2 nuclear localization in sections of rat hindbrain and dorsal root ganglia tissue, using two anti-Kir2.2 polyclonal antisera with different epitope specificities. These data confirm nuclear localization and are suggestive of new functions of Kir2.0 potassium ion channels in the nucleus.
- Atherton E. and Sheppard R. C. (1985) Solid-phase peptide-synthesis using N-alpha-fluorenylmethoxy-carbonylamino acid pentafluorophenyl esters. J. Chem. Soc. 3, 165–166.
- Bootman M. D., Thomas D., Tovey S. C., Berridge M. J., and Lipp P. (2000) Nuclear calcium signalling. Cell. Mol. Life Sci. 57, 371–378. CrossRef
- Draguhn A., Borner G., Beckmann R., Buchner K., Heinemann U., and Hucho F. (1997) Large-conductance cation channels in the envelope of nuclei from rat cerebral cortex. J. Mem. Biol. 158, 159–166. CrossRef
- Gerasimenko O. V., Gerasimenko J. V., Tepikin A. V., and Petersen O. H. (1995) ATP-dependent accumulation and inositol triphosphate- or cyclic ADP-ribose-mediated release of Ca2+ from the nuclear envelope. Cell 80, 439–444. CrossRef
- Gerasimenko O. V., Gerasimenko J. V., Tepikin A. V., and Petersen O. H. (1996) Calcium transport pathways in the nucleus Pflügers Archiv. 432, 1–6. CrossRef
- Hille B. (1992) Ionic Channels of Excitable Membranes, 2nd ed., Sinauer, Sunderland, MA.
- Jaimovich E., Reyes R., Liberona J. L., and Powell J. A. (2000) IP3 receptors, IP3 tansients, and nucleus-associated Ca2+ signals in culture skeletal muscle. Am. J. Physiol. 278, C998-C1010.
- Karschin C., Dissmann E., Stühmer W., and Karschin A. (1996) IRK(1–3) and GIRK(1–4) inwardly rectifying K+ channel mRNAs are differentially expressed in the adult rat brain. J. Neurosci. 16, 3559–3570.
- LaRochelle W. J., Wray B. E., Sealock R., and Froehner S. C. (1985) Immunochemical demonstration that amino-acids 360–377 of the acetylcholine-receptor γ-subunit are cytoplasmic. J. Cell Biol. 100, 684–691. CrossRef
- Maruyama Y., Shimada H., and Taniguchi J. (1995) Ca2+-activated K+-channels in the nuclear envelope isolated from single pancreatic acinar cells. Pflügers Archiv. 430, 148–150. CrossRef
- Mi H., Deerinck T. J., Jones M., Ellisman M. H., and Schwarz T. L. (1996) Inwardly rectifying K+ channels that may participate in K+ buffering are localized in microvilli of Schwann cells. J. Neurosci. 16, 2421–2429.
- Raab-Graham K. F. and Vandenberg C. A. (1998) Tetrameric subunit structure of the native brain inwardly rectifying potassium channel Kir2.2. J. Biol. Chem. 273, 19699–19707. CrossRef
- Stanfield P. R., Nakajima S., and Nakajima Y. (2002) Constitutively active and G-protein coupled inward rectifier K+ channels: Kir2.0 and Kir3.0. Rev. Physiol. Biochem. Pharmacol. 145, 47–179. CrossRef
- Stonehouse A. H., Pringle J. H., Norman R. I. Stanfield P. R., Conley E. C., and Brammar W. J. (1999a) Demonstration of inwardly rectifying potassium ion channel, Kir2.2, and substance P receptor expression in the CNS using polyclonal antibodies. J. Physiol. 518P, 129.
- Stonehouse A. H., Pringle J. H., Norman R. I., Stanfield P. R., Conley E. C., and Brammar W. J. (1999b) Characterisation of Kir2.0 proteins in the rat cerebellum and hippocampus by polyclonal antibodies. Histochem. Cell. Biol. 112, 457–465. CrossRef
- Wallinga W., Meijer S. L., Alberink M. J., Vliek M., Wienk E. D., and Ypey D. L. (1999) Modelling action potentials and membrane currents of mammalian skeletal muscle fibres in coherence with potassium concentration changes in the T-tubular system. Eur. Biophys. J. 28, 317–329. CrossRef
- Nuclear immunostaining in rat neuronal cells using two anti-kir2.2 ion channel polyclonal antibodies
Journal of Molecular Neuroscience
Volume 20, Issue 2 , pp 189-193
- Cover Date
- Print ISSN
- Online ISSN
- Humana Press
- Additional Links
- affinity-purified polyclonal antibody
- nuclear staining
- fastigial nuclei
- vestibular nuclei
- dorsal root ganglia
- Industry Sectors
- Author Affiliations
- 1. The Neurosciences Institute, 10640 John Jay Hopkins Drive, 92121, San Diego, CA, USA
- 2. Department of Cell Physiology and Pharmacology, University of Leicester, Maurice Shock Building, LE1 9HN, Leicester, UK
- 3. Department of Pathology, University of Leicester, Robert Kilpatrick Building, LE2 7LX, Leicester, UK
- 4. Department of Medicine, University of Leicester, Robert Kilpatrick Building, LE2 7LX, Leicester, UK
- 5. Department of Biological Sciences, University of Warwick, Gibbet Hill Road, CV4 7AL, Coventry, UK
- 6. Department of Biochemistry, University of Leicester, Adrian Building, LE1 7RH, Leicester, UK