Journal of Molecular Neuroscience

, Volume 20, Issue 2, pp 189–193

Nuclear immunostaining in rat neuronal cells using two anti-kir2.2 ion channel polyclonal antibodies

  • Anthony H. Stonehouse
  • Blair D. Grubb
  • J. Howard Pringle
  • Robert I. Norman
  • Peter R. Stanfield
  • William J. Brammar
Brief Communication

DOI: 10.1385/JMN:20:2:189

Cite this article as:
Stonehouse, A.H., Grubb, B.D., Pringle, J.H. et al. J Mol Neurosci (2003) 20: 189. doi:10.1385/JMN:20:2:189

Abstract

The inwardly rectifying potassium ion channel Kir2.2 has recently been demonstrated to have nuclear and plasma membrane subcellular localization. Nuclear expression of Kir2.2 is controversial, as a functional role for Kir2.0 potassium channels in the nucleus has not been investigated. However, in this report we have demonstrated Kir2.2 nuclear localization in sections of rat hindbrain and dorsal root ganglia tissue, using two anti-Kir2.2 polyclonal antisera with different epitope specificities. These data confirm nuclear localization and are suggestive of new functions of Kir2.0 potassium ion channels in the nucleus.

Index Entries

Kirk2.2affinity-purified polyclonal antibodynuclear stainingfastigial nucleivestibular nucleidorsal root ganglia

Copyright information

© Humana Press Inc 2003

Authors and Affiliations

  • Anthony H. Stonehouse
    • 1
  • Blair D. Grubb
    • 2
  • J. Howard Pringle
    • 3
  • Robert I. Norman
    • 4
  • Peter R. Stanfield
    • 5
  • William J. Brammar
    • 6
  1. 1.The Neurosciences InstituteSan DiegoUSA
  2. 2.Department of Cell Physiology and PharmacologyUniversity of LeicesterLeicesterUK
  3. 3.Department of PathologyUniversity of LeicesterLeicesterUK
  4. 4.Department of MedicineUniversity of LeicesterLeicesterUK
  5. 5.Department of Biological SciencesUniversity of WarwickCoventryUK
  6. 6.Department of BiochemistryUniversity of LeicesterLeicesterUK