International Journal of Pancreatology

, Volume 26, Issue 3, pp 147–154

The endothelin antagonist bosentan does not improve survival in severe experimental pancreatitis in rats

  • F. Fiedler
  • D. Ayasse
  • P. Rohmeiss
  • N. Gretz
  • C. Rehbein
  • V. Keim
Original Article

DOI: 10.1385/IJGC:26:3:147

Cite this article as:
Fiedler, F., Ayasse, D., Rohmeiss, P. et al. International Journal of Pancreatology (1999) 26: 147. doi:10.1385/IJGC:26:3:147

Summary

Background. Severity of pancreatitis seems to be aggravated by impairment of vascular perfusion of the gland. Early mortality occurs within the first few days from the acute consequences of pancreatic injury with subsequent inflammatory response. Because vasoactive substances, including endothelin, seem to contribute to early mortality in acute pancreatitis, we tested the hypothesis that the inhibition of endothelin action could alter the outcome after severe experimental pancreatitis.

Methods. In two groups of rats, pancreatitis was induced by intraductal infusion into the pancreatic duct of 1 µL/g body weight (b.w.) of either a 4% or a 5% sodium taurocholate solution. The mixed endothelin A and endothelin B receptor antagonist bosentan (20 mg/kg b.w.) or vehicle was injected intravenously in 12-h intervals for 3 d starting 1 h after induction of bile acid pancreatitis. This dose of bosentan is known to completely inhibit the effect of exogenous endothelin. The survival rate was monitored for 7 d. Thereafter, the surviving rats were sacrificed and the pancreas was prepared for histological and biochemical evaluation.

Results. Irrespective of the treatment protocol (bosentan versus saline), survival was not different in animals challenged with either 4% or 5% sodium taurocholate. The corresponding survival rates were 62% with bosentan and 77% without bosentan in the 4% sodium taurocholate group. In the 5% sodium taurocholate group, the survival rates were 20% with and 27% without bosentan. Morphological and biochemical alterations were identical in control as well as in endothelin-antagonist-treated rats.

Conclusion. Therapy with the mixed endothelin A and endothelin B receptor antagonist bosentan does not influence the outcome after severe experimental pancreatitis. Therefore, blockade of endothelin A and B receptor subtypes may not be of major importance as a therapeutic principle in this model of experimental pancreatitis.

Key Words

Acute experimental pancreatitisendothelinendothelin-receptor antagonistbosentan

Copyright information

© Humana Press Inc 1999

Authors and Affiliations

  • F. Fiedler
    • 5
  • D. Ayasse
    • 5
  • P. Rohmeiss
    • 1
  • N. Gretz
    • 2
  • C. Rehbein
    • 3
  • V. Keim
    • 4
  1. 1.Medizinische Klinik V, Klinikum MannheimUniversität HeidelbergMannheimGermany
  2. 2.Zentrum für Medizinische Forschung, Klinikum MannheimUniversität HeidelbergMannheimGermany
  3. 3.Institut für Pathologie, Klinikum MannheimUniversität HeidelbergMannheimGermany
  4. 4.Medizinische Klinik und Poliklinik IIUniversität LeipzigGermany
  5. 5.Institut für Anästhesiologie und Operative Intensivmedizin, Klinikum MannheimFakultät für Klinische Medizin Mannheim der Universität Heidelberg, Theodor-Kutzer UferMannheimGermany