Original Articles

Endocrine

, Volume 30, Issue 1, pp 93-101

Effects of pueraria mirifica, an herb containing phytoestrogens, on reproductive organs and fertility of adult male mice

  • Sukanya JaroenpornAffiliated withInterdepartment of Physiology, Faculty of Graduate School, Chulalongkorn UniversityPrimate Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University
  • , Suchinda MalaivijitnondAffiliated withPrimate Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University Email author 
  • , Kingkaew WattanasirmkitAffiliated withPrimate Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University
  • , Hataitip TrisomboonAffiliated withDepartment of Physiology, Faculty of Medicine, Srinakharinwirot UniversityLaboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • , Gen WatanabeAffiliated withLaboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and TechnologyDepartment of Basic Veterinary Science, The United Graduated School of Veterinary Sciences, Gifu University
  • , Kazuyoshi TayaAffiliated withLaboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and TechnologyDepartment of Basic Veterinary Science, The United Graduated School of Veterinary Sciences, Gifu University
  • , Wichai CherdshewasartAffiliated withPrimate Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University

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Abstract

The effects of Pueraria mirifica (PM) on reproductive organs and fertility of adult male mice were investigated. Male mice were divided into four groups (10 mice/group). Groups 1–3 were orally treated with PM at doses of 0 (PM-0), 10 (PM-10), and 100 (PM-100) mg/kg BW/d in 0.2 mL distilled water, and group 4 was subcutaneously injected with 200 μg/kg BW/d of synthetic estrogen diesthylstibestol (DES). The treatment schedule was separated into two periods: treatment and posttreatment (8 wk for each period). The PM-10 and PM-100 treatments had no effect on testicular weight, sperm number, and serum LH, FSH, and testosterone levels. Only the PM-100 treatment reduced weights of epididymes and seminal vesicle and the sperm motility and viability. Histopathological examination demonstrated that testis, epididymis, and seminal vesicle were normal in all doses of PM treatment. PM-treated males showed no alterations in mating efficiency and on causing pregnancy of their female partners. DES injection impaired all those parameters. Offspring fathered by the PM-and DES-treated males exhibited neither malformations nor change of body weight gains, and the reproductive organ weights of 50-d old pups were in the normal range. The present data clearly demonstrate that a long-term treatment of PM at doses 10 and 100 mg/kg BW/d, via oral route, does not alter a male fertility and a hypothalamus pituitary-testis axis. Although PM-100 can cause some moderate impairment, no persistent effects were observed. Most of PM-treated mice increased the mating efficiency after stop treatment.

Key Words

Diethylstibestrol male mice Pueraria mirifica gonadotropins sperm quality testis