Article

Endocrine

, Volume 17, Issue 3, pp 185-191

First online:

Glucose intolerance induced by a high-fat/low-carbohydrate diet in rats

Effects of nonesterified fatty acids
  • Yuan WangAffiliated withDepartment of Environmental Health, Medical University of Yamanashi, Tamaho
  • , Yoshikazu MiuraAffiliated withDepartment of Hygiene, Dokkyo University School of Medicine, Mibu
  • , Takashi KanekoAffiliated withDepartment of Environmental Health, Medical University of Yamanashi, Tamaho Email author 
  • , Jue LiAffiliated withDepartment of Environmental Health, Medical University of Yamanashi, Tamaho
  • , Li-Qiang QinAffiliated withDepartment of Environmental Health, Medical University of Yamanashi, Tamaho
  • , Pei-Yu WangAffiliated withDepartment of Environmental Health, Medical University of Yamanashi, Tamaho
  • , Hisao MatsuiAffiliated withDepartment of Hygiene, Dokkyo University School of Medicine, Mibu
  • , Akio SatoAffiliated withDepartment of Environmental Health, Medical University of Yamanashi, Tamaho

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Abstract

We examined the time course of effects of a high-fat/low-carbohydrate (HF/LC) diet on the impairment of glucose tolerance in rats, clarified whether insulin secretion and sensitivity were impaired by the HF/LC diet, and investigated the relationship between the increased nonesterified fatty acids (NEFA) after HF/LC diet feeding and insulin secretion and sensitivity. We found that glucose tolerance and the postglucose-loading insulin secretion were impaired after 3 and 7 d on the HF/LC diet. The glucose intolerance was accompanied by a rise in the fasting plasma NEFA level. When stimulated with 15 mmol/L of glucose, the insulin secretion was impaired in pancreatic islets from rats fed the HF/LC diet. Rats fed the HF/LC diet showed insulin resistance in vivo. The glucose-stimulated insulin secretion was inhibited in the islets following 24-h culture with palmitic acid. The 24-h infusion of palmitic acid decreased whole-body insulin sensitivity. In summary, at least 3 d on a HF/LC diet is needed to induce glucose intolerance in rats, and the impairment may be induced by decreased insulin secretion and sensitivity, which is related to the increase in the plasma NEFA level.

Key Words

High-fat/low-carbohydrate diet glucose intolerance insulin secretion insulin sensitivity non-esterified fatty acids