Original Article

Cell Biochemistry and Biophysics

, Volume 41, Issue 3, pp 343-356

First online:

Visualization of the uptake of individual HDL particles in living cells via the scavenger receptor class B type I

  • Sebastian RhodeAffiliated withInstitute for Biophysics, University of Linz
  • , Anton BreuerAffiliated withInstitute for Biophysics, University of Linz
  • , Jan HesseAffiliated withInstitute for Biophysics, University of Linz
  • , Max SonnleitnerAffiliated withCenter for Biomedical Nanotechnology, Upper Austrian Research GmbH
  • , Tamara A. PaglerAffiliated withInstitute for Medical Chemistry, Medical University of Vienna
  • , Marlon DoringerAffiliated withInstitute for Medical Chemistry, Medical University of Vienna
  • , Gerhard J. SchützAffiliated withInstitute for Biophysics, University of Linz Email author 
  • , Herbert StanglAffiliated withInstitute for Medical Chemistry, Medical University of Vienna Email author 

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Abstract

The scavenger receptor class B type I (SR-BI) plays an important role in mediating selective uptake of high-density lipoprotein (HDL)-derived cholesterol and cholesteryl ester in liver and steroidogenic tissues. The molecular mechanism by which this receptor mediates selective cholesteryl ester uptake remains still enigmatic. We applied ultrasensitive fluorescence microscopy to visualize the intracellular transport routes of HDL particles taken up via SR-BI in a Chinese hamster ovarian cell line. Although diffusion of the receptor bound particles on the cell surface is slow, internalization is accompanied by a dramatic increase in the mobility of the particles. HDL particles are endocytosed as clusters and actively transported to the perinuclear region of the cell. Costaining with organelle markers confirmed the involvement of an acidic compartment and the Golgi apparatus in the uptake process; finally, resecretion of the HDL particles was observed.

Index Entries

SR-BI HDL uptake selective cholesteryl ester uptake ultrasensitive microscopy retro-endocytosis chinese hamster ovarian cells