Cell Biochemistry and Biophysics

, Volume 41, Issue 3, pp 343–356

Visualization of the uptake of individual HDL particles in living cells via the scavenger receptor class B type I

Authors

  • Sebastian Rhode
    • Institute for BiophysicsUniversity of Linz
  • Anton Breuer
    • Institute for BiophysicsUniversity of Linz
  • Jan Hesse
    • Institute for BiophysicsUniversity of Linz
  • Max Sonnleitner
    • Center for Biomedical NanotechnologyUpper Austrian Research GmbH
  • Tamara A. Pagler
    • Institute for Medical ChemistryMedical University of Vienna
  • Marlon Doringer
    • Institute for Medical ChemistryMedical University of Vienna
    • Institute for BiophysicsUniversity of Linz
    • Institute for Medical ChemistryMedical University of Vienna
Original Article

DOI: 10.1385/CBB:41:3:343

Cite this article as:
Rhode, S., Breuer, A., Hesse, J. et al. Cell Biochem Biophys (2004) 41: 343. doi:10.1385/CBB:41:3:343

Abstract

The scavenger receptor class B type I (SR-BI) plays an important role in mediating selective uptake of high-density lipoprotein (HDL)-derived cholesterol and cholesteryl ester in liver and steroidogenic tissues. The molecular mechanism by which this receptor mediates selective cholesteryl ester uptake remains still enigmatic. We applied ultrasensitive fluorescence microscopy to visualize the intracellular transport routes of HDL particles taken up via SR-BI in a Chinese hamster ovarian cell line. Although diffusion of the receptor bound particles on the cell surface is slow, internalization is accompanied by a dramatic increase in the mobility of the particles. HDL particles are endocytosed as clusters and actively transported to the perinuclear region of the cell. Costaining with organelle markers confirmed the involvement of an acidic compartment and the Golgi apparatus in the uptake process; finally, resecretion of the HDL particles was observed.

Index Entries

SR-BIHDL uptakeselective cholesteryl ester uptakeultrasensitive microscopyretro-endocytosischinese hamster ovarian cells

Copyright information

© Humana Press Inc 2004