Cell Biochemistry and Biophysics

, Volume 40, Supplement 3, pp 179–190

Imaging glucose-regulated insulin secretion and gene expression in single islet β-cells

Control by AMP-activated protein kinase
  • Guy A. Rutter
  • Isabelle Leclerc
  • Takashi Tsuboi
  • Gabriela da Silva Xavier
  • Frédérique Diraison
  • Qingwen Qian
Pancreatic And Islet Cell Biology

DOI: 10.1385/CBB:40:3:179

Cite this article as:
Rutter, G.A., Leclerc, I., Tsuboi, T. et al. Cell Biochem Biophys (2004) 40(Suppl 3): 179. doi:10.1385/CBB:40:3:179
  • 81 Downloads

Abstract

The mechanisms by which changes in glucose concentration regulate gene expression and insulin secretion in pancreatic islet β-cells are only partly understood. Here we describe the development of new technologies for examining these processes at the level of single living β-cells. We also present recent findings, made using these and other techniques, which implicate a role for adenosine 5′-monophosphate-activated protein kinase in glucose signaling in these cells.

Index Entries

Insulin islet type 2 diabetes secretion AMP-activated protein kinase evanescent wave/total internal reflection fluorescence bioluminescence imaging microinjection 

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Guy A. Rutter
    • 1
  • Isabelle Leclerc
    • 1
  • Takashi Tsuboi
    • 1
  • Gabriela da Silva Xavier
    • 1
  • Frédérique Diraison
    • 1
  • Qingwen Qian
    • 1
  1. 1.Henry Wellcome Laboratories of Integrated Cell Signalling and Department of Biochemistry, School of Medical Sciences, University WalkUniversity of BristolUnited Kingdom

Personalised recommendations