Cell Biochemistry and Biophysics

, Volume 40, Issue 2, pp 225–236

Calcium signaling in isolated skeletal muscle fibers investigated under “silicone voltage-clamp” conditions

  • Claude Collet
  • Sandrine Pouvreau
  • Laszlo Csernoch
  • Bruno Allard
  • Vincent Jacquemond
Review Article

DOI: 10.1385/CBB:40:2:225

Cite this article as:
Collet, C., Pouvreau, S., Csernoch, L. et al. Cell Biochem Biophys (2004) 40: 225. doi:10.1385/CBB:40:2:225

Abstract

In skeletal muscle, release of calcium from the sarcoplasmic reticulum (SR) represents the major source of cytoplasmic Ca2+ elevation. SR calcium release is under the strict command of the membrane potential, which drives the interaction between the voltage sensors in the t-tubule membrane and the calcium-release channels. Either detection or control of the membrane voltage is thus essential when studying intracellular calcium signaling in an intact muscle fiber preparation. The silicone-clamp technique used in combination with intracellular calcium measurements represents an efficient tool for such studies. This article reviews some properties of the plasma membrane and intracellular signals measured with this methodology in mouse skeletal muscle fibers. Focus is given to the potency of this approach to investigate both fundamental aspects of excitation-contraction coupling and potential alterations of intracellular calcium handling in some muscle diseases.

Index Entries

Skeletal muscle voltage-clamp calcium channel voltage-sensor intracellular calcium SR calcium release muscle dystrophy 

Copyright information

© Humana Press Inc 2004

Authors and Affiliations

  • Claude Collet
    • 1
  • Sandrine Pouvreau
    • 1
  • Laszlo Csernoch
    • 2
  • Bruno Allard
    • 1
  • Vincent Jacquemond
    • 1
  1. 1.Laboratoire de Physiologie des Eléments ExcitablesUniversité Claude Bernard Lyon 1, UMR CNRS 5123VilleurbanneFrance
  2. 2.Department of Physiology; Health Sciences CentreUniversity of DebrecenDebrecenHungary