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Effects of dietary chromium chloride supplementation on performance, some serum parameters, and immune response in broilers

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Abstract

This study was conducted to investigate the effect of increasing dietary levels of inorganic chromium (CrCl3·6H2O) on the performance, blood chemistry, and immune response of broilers. Eighty newly hatched Ross PM3 broiler chicks were evenly distributed to five groups of 16 chicks each. Two groups (control and only sheep red blood cell inoculated) were fed the basal diet containing 2.2 and 4.5 mg Cr/kg and the remaining groups were fed 20, 40, or 80 mg/kg Cr-supplemented diets for 44 d. Chicks in all groups, except in the control, at 3 and 5 wk of age, were injected intraperitonally with sheep red blood cell for determining the primary and secondary antibody responses, respectively. When the chicks were 4 wk of age, a delayed-type hypersensitivity test was performed. White blood cells were differentiated. Blood samples were collected for the determination of serum proteins, glucose, cholesterol, cortisol, minerals, and alkaline phosphatase activity and for antibody response. Chromium had no effect on weight gain, but 20 mg/kg supplemental Cr resulted in 18.57% reduction in feed consumption and improved feed efficiency by 16.77%. Chromium did not affect serum cholesterol and P levels but reduced serum glucose and increased serum protein, Cr, Ca, and Mg levels, and ALP activity. A slight reduction was observed with Cr supplementation in cortisol levels. Slight but not significant increases were observed with Cr in serum Zn and Cu. Chromium increased the ratio of bursa of Fabricius and liver to body weight. Heterophil and monocyte counts and heterophil/lymphocyte ratio were reduced and lymphocyte counts, total antibody, IgG, and IgM titers were increased by supplemental Cr. All levels of Cr increased the cell-mediated response to phytohemagglutinin. No alterations in tissues were observed by histopathological examinations.

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Authors and Affiliations

  1. Department of Biochemistry, University of Erciyes, Kayseri, Turkey

    Fatma Uyanik

  2. Department of Pathology, University of Erciyes, Kayseri, Turkey

    Ayhan Atasever

  3. Department of Microbiology, Faculty of Veterinary Medicine, University of Erciyes, Kayseri, Turkey

    Fuat Aydin

  4. Department of Histology, Faculty of Medicine, University of Erciyes, Kayseri, Turkey

    Saim Özdamar

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  1. Fatma Uyanik
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  3. Saim Özdamar
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  4. Fuat Aydin
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Uyanik, F., Atasever, A., Özdamar, S. et al. Effects of dietary chromium chloride supplementation on performance, some serum parameters, and immune response in broilers. Biol Trace Elem Res 90, 99–115 (2002). https://doi.org/10.1385/BTER:90:1-3:99

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  • DOI: https://doi.org/10.1385/BTER:90:1-3:99

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