Applied Biochemistry and Biotechnology

, Volume 96, Issue 1, pp 303–311

Immobilization of lactate dehydrogenase on tetraethylorthosilicate-derived sol-gel films for application to lactate biosensor

Authors

    • Biomolecular Electronics and Conducting Polymer Research GroupNational Physical Laboratory
    • Department of BiochemistryL.L.R.M. Medical College
  • Manju Gerard
    • Biomolecular Electronics and Conducting Polymer Research GroupNational Physical Laboratory
  • V. S. Singh
    • Department of BiochemistryL.L.R.M. Medical College
  • B. D. Malhotra
    • Biomolecular Electronics and Conducting Polymer Research GroupNational Physical Laboratory
Article

DOI: 10.1385/ABAB:96:1-3:303

Cite this article as:
Chaubey, A., Gerard, M., Singh, V.S. et al. Appl Biochem Biotechnol (2001) 96: 303. doi:10.1385/ABAB:96:1-3:303

Abstract

Tetraethylorthosilicate (TEOS)-derived sol-gel films were utilized for the immobilization of lactate dehydrogenase (LDH) by physical adsorption and sol-gel/LDH/sol-gel sandwich configuration. An attempt was made to ascertain the optimum pH and temperature for the immobilized LDH. It was shown that TEOS-derived sol-gel films containing physically adsorbed LDH exhibited linearity from 0.5 to 4 mM, whereas those containing LDH in sandwich configuration showed linearity from 0.5 to 3 mM l-lactate. These sol-gel films, immobilized with LDH, were found to be stable for about 4 weeks at 4–10°C.

Index Entries

Immobilization lactate dehydrogenase tetraethylorthosilicate (TEOS) sol-gel lactate biosensor physical adsorption sandwich configuration

Copyright information

© Humana Press Inc. 2001