Immobilization of lactate dehydrogenase on tetraethylorthosilicate-derived sol-gel films for application to lactate biosensor
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- Chaubey, A., Gerard, M., Singh, V.S. et al. Appl Biochem Biotechnol (2001) 96: 303. doi:10.1385/ABAB:96:1-3:303
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Tetraethylorthosilicate (TEOS)-derived sol-gel films were utilized for the immobilization of lactate dehydrogenase (LDH) by physical adsorption and sol-gel/LDH/sol-gel sandwich configuration. An attempt was made to ascertain the optimum pH and temperature for the immobilized LDH. It was shown that TEOS-derived sol-gel films containing physically adsorbed LDH exhibited linearity from 0.5 to 4 mM, whereas those containing LDH in sandwich configuration showed linearity from 0.5 to 3 mMl-lactate. These sol-gel films, immobilized with LDH, were found to be stable for about 4 weeks at 4–10°C.