Applied Biochemistry and Biotechnology

, Volume 79, Issue 1, pp 649–665

Simultaneous saccharification and cofermentation of dilute-acid pretreated yellow poplar hardwood to ethanol using xylose-fermenting Zymomonas mobilis

  • James D. McMillan
  • Mildred M. Newman
  • David W. Templeton
  • Ali Mohagheghi
Article

DOI: 10.1385/ABAB:79:1-3:649

Cite this article as:
McMillan, J.D., Newman, M.M., Templeton, D.W. et al. Appl Biochem Biotechnol (1999) 79: 649. doi:10.1385/ABAB:79:1-3:649

Abstract

Simultaneous saccharification and cofermentation (SSCF) was carried out at approximately 15% total solids using conditioned dilute-acid pretreated yellow poplar feedstock, an adapted variant of National Renewable Energy Laboratory (NREL) xylose-fermenting Zymomonas mobilis and either commercial or NREL-produced cellulase enzyme preparations. In 7 d, at a cellulase loading of 12 filter paper units pergram cellulose (FPU/g), the integrated system produced more than 3% w/v ethanol and achieved 54% conversion of all potentially available biomass sugars (total sugars) entering SSCF. A control SSCF employing Sigmacell cellulose and a commercial cellulase at an enzyme loading of 14 FPU/gachieved 65% conversion of total sugars to ethanol.

Index Entries

Hydrolyzate conditioningZymomonas mobifisenzymatic saccharificationethanol fermentationSSCFintegrated testing

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • James D. McMillan
    • 1
  • Mildred M. Newman
    • 1
  • David W. Templeton
    • 1
  • Ali Mohagheghi
    • 1
  1. 1.Biotechnology Center for Fuels and ChemicalsNational Renewable Energy LaboratoryGolden