Accumulation of biopolymers in activated sludge biomass
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In this study, activated sludge bacteria from a conventional wastewater treatment process were induced to accumulate polyhydroxyalkanoates (PHAs) under different carbon-nitrogen (C:N) ratios. As the C:N ratio increased from 20 to 140, specific polymer yield increased to a maximum of 0.38 g of polymer/g of dry cell mass while specific growth yield decreased. The highest overall polymer production yield of 0.11 g of polymer/g of carbonaceous substrate consumed was achieved using a C:N ratio of 100. Moreover, the composition of polymer accumulated was dependent on the valeric acid content in the feed. Copolymer poly (3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] was produced in the presence of valeric acid. The 3-hydroxyvalerate (3HV) mole fraction in the copolymer was linearly related tovaleric content in the feed, which reached a maximum of 54% when valeric acid was used as sole carbon source. When the 3HV U in the polymer increased from 0–54 mol%, the melting temperature decreased from 178° to 99°C. Thus, the composition, and hence the mechanical properties, of the copolymer produced from activated sludge can be controlled by adjusting the mole fraction of valeric acid in the feed medium.
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- Accumulation of biopolymers in activated sludge biomass
Applied Biochemistry and Biotechnology
Volume 78, Issue 1-3 , pp 389-399
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- Humana Press
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- Activated sludge
- carbon-nitrogen ratio
- wastewater treatment
- butyric-valeric acid ratio
- Industry Sectors
- Author Affiliations
- 1. Department of Civil and Structural Engineering, The Hong Kong Polytechnic University, Kowloon, Hung Hom, Hong Kong, China
- 2. Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon, Hung Hom, Hong Kong, China