Applied Biochemistry and Biotechnology

, Volume 130, Issue 1, pp 599–611

Optimal conditions for alkaline detoxification of dilute-acid lignocellulose hydrolysates


    • Biochemistry, Division for ChemistryKarlstad University
  • Anders Sjöde
    • Biochemistry, Division for ChemistryKarlstad University
  • Nils-Olof Nilvebrant
    • STFI-Packforsk
  • Leif J. Jönsson
    • Biochemistry, Division for ChemistryKarlstad University
Session 2 Today's Biorefineries

DOI: 10.1385/ABAB:130:1:599

Cite this article as:
Alriksson, B., Sjöde, A., Nilvebrant, N. et al. Appl Biochem Biotechnol (2006) 130: 599. doi:10.1385/ABAB:130:1:599


Alkaline detoxification strongly improves the fermentability of dilute-acid hydrolysates in the production of bioethanol from lignocellulose with Saccharomyces cerevisiae. New experiments were performed with NH4OH and NaOH to define optimal conditions for detoxification and make a comparison with Ca(OH)2 treatment feasible. As too harsh conditions lead to sugar degradation, the detoxification treatments were evaluated through the balanced ethanol yield, which takes both the ethanol production and the loss of fermentable sugars into account. The optimization treatments were performed as factorial experiments with 3-h duration and varying pH and temperature. Optimal conditions were found roughly in an area around pH 9.0/60°C for NH4OH treatment and in a narrow area stretching from pH 9.0/80°C to pH 12.0/30°C for NaOH treatment. By optimizing treatment with NH4OH, NaOH, and Ca(OH)2, it was possible to find conditions that resulted in a fermentability that was equal or better than that of a reference fermentation of a synthetic sugar solution without inhibitors, regardless of the type of alkali used. The considerable difference in the amount of precipitate generated after treatment with different types of alkali appears critical for industrial implementation.

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© Humana Press Inc 2006