Applied Biochemistry and Biotechnology

, Volume 115, Issue 1, pp 1103–1114

Enhanced enzymatic hydrolysis of steam-exploded douglas fir wood by alkali-oxygen post-treatment

  • Xuejun Pan
  • Xiao Zhang
  • David J. Gregg
  • John N. Saddler
Session 6A Biomass Pretreatment and Hydrolysis

DOI: 10.1385/ABAB:115:1-3:1103

Cite this article as:
Pan, X., Zhang, X., Gregg, D.J. et al. Appl Biochem Biotechnol (2004) 115: 1103. doi:10.1385/ABAB:115:1-3:1103

Abstract

Good enzymatic hydrolysis of steam-exploded Douglas fir wood (SEDW) cannot be achieved owing to the very high lignin content (>40%) that remains associated with this substrate. Thus, in this study, we investigated the use of alkali-oxygen treatment as a posttreatment to delignify SEDW and also considered the enzymatic hydrolyzability of the delignified SEDW. The results showed that under optimized conditions of 15% NaOH, 5% consistency, 110°C, and 3h, approx84% of the lignin in SEDW could be removed. The resulting delignified SEDW had good hydrolyzability, and cellulose-to-glucose conversion yields of over 90 and 100% could be achieved within 48 h with 20 and 40 filter paper units/g of cellulose enzyme loadings, respectively. It was also indicated that severe conditions, such as high NaOH concentration and high temperature, should not be utilized in oxygen delignification of SEDW in order to avoid extensive condensation of lignin and significant degradation of cellulose.

Index Entries

Steam explosionDouglas firoxygen delignificationenzymatic hydrolysislignin

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Xuejun Pan
    • 1
  • Xiao Zhang
    • 1
  • David J. Gregg
    • 1
  • John N. Saddler
    • 1
  1. 1.Forest Products Biotechnology, Faculty of ForestryUniversity of British ColumbiaVancouverCanada