Applied Biochemistry and Biotechnology

, Volume 115, Issue 1, pp 989–1002

Combined steam pretreatment and enzymatic hydrolysis of starch-free wheat fibers


  • Beatriz Palmarola-Adrados
    • Department of Chemical Engineering 1Lund University
  • Mats Galbe
    • Department of Chemical Engineering 1Lund University
    • Department of Chemical Engineering 1Lund University
Session 6A Biomass Pretreatment and Hydrolysis

DOI: 10.1385/ABAB:115:1-3:0989

Cite this article as:
Palmarola-Adrados, B., Galbe, M. & Zacchi, G. Appl Biochem Biotechnol (2004) 115: 989. doi:10.1385/ABAB:115:1-3:0989


Steam treatment of an industrial process stream, denoted starch-free wheat fiber, was investigated to improve the formation of monomeric sugars in subsequent enzymatic hydrolysis for further bioconversion into ethanol. The solid fraction in the process stream, derived from a combined starch and ethanol factory, was rich in arabinose (21.1%), xylose (30.1%), and glucose (18.6%), in the form of polysaccharides. Various conditions of steam pretreatment (170–220°C for 5–30 min) were evaluated, and their effect was assessed by enzymatic hydrolysis with 2 g of Celluclast + Ultraflo mixture/ 100 g of starch-free fiber (SFF) slurry at 5% dry matter (DM). The highest overall sugar yield for the combined steam pretreatment and enzymatic hydrolysis, 52g/100 g of DM of SFF, corresponding to 74% of the theoretical, was achieved with pretreatment at 190°C for 10 min followed by enzymatic hydrolysis.

Index Entries

Wheat fiberssteam pretreatmentenzymatic hydrolysisstarch-free fibermicrowave oven

Copyright information

© Humana Press Inc. 2004