Applied Biochemistry and Biotechnology

, Volume 114, Issue 1, pp 403–416

Production of ethanol from cellulosic biomass hydrolysates using genetically engineered saccharomyces yeast capable of cofermenting glucose and xylose

Session 2—Introduction to Microbial Catalysis and Engineering

DOI: 10.1385/ABAB:114:1-3:403

Cite this article as:
Sedlak, M. & Ho, N.W.Y. Appl Biochem Biotechnol (2004) 114: 403. doi:10.1385/ABAB:114:1-3:403


Recent studies have proven ethanol to be the idael liquid fuel for transportation, and renewable ligno cellulosic materials to be the attractive feed stocks for ethanol fuel production by fermentation. The major fermentable sugars from hydrolysis of most cellulosic biomass are D-glucose and D-xylose. The naturally occurring Saccharomyces yeasts that are used by industry to produce ethanol from starches and cane sugar cannot metabolize xylose. Our group at Purdue University succeded in developing genetically engineered Saccharomyces yeasts capable of effectively cofermenting glucose and xylose to ethanol, which was accomplished by cloning three xylose-metabolizing genes into the yeast. In this study, we demonstrated that our stable recombinant Sacharomyces yeast, 424A (LNH-ST), which contains the cloned xylose-metabolizing genes stably integrated into the yeast chromosome in high copy numbers, can efficiently ferment glucose and xylose present in hydrolysates from different cellulosic biomass to ethanol.

Index Entries

EthanolSaccharomyces yeastshydrolysatecorn stovercorn fiberxyloseglucoseglycerolxylitol

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  1. 1.Laboratory of Renewable Resources Engineering (LORRE)Purde UniversityWest Lafayette