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Magnetite-alginate beads for purification of some starch degrading enzymes

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Abstract

Starch degrading enzymes, viz., β-amylase, glucoamylase, and pullulanase, were purified using magnetite-alginate beads. In each case, the enzyme activity was eluted by using 1.0 M maltose. β-Amylase (sweet potato), glucoamylase (Aspergillus niger), and pullulanase (Bacillus acidopullulyticus) from their crude preparations were purified 37-, 31-, and 49-fold with 86, 87, and 95% activity recovery, respectively.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed single band in each case.

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Authors and Affiliations

  1. Chemistry Department, Indian Institute of Technology, Hauz Khas, 110016, New Delhi, India

    M. N. Gupta

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  1. Sunita Teotia
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  2. M. N. Gupta
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Correspondence to M. N. Gupta.

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Teotia, S., Gupta, M.N. Magnetite-alginate beads for purification of some starch degrading enzymes. Mol Biotechnol 20, 231–237 (2002). https://doi.org/10.1385/MB:20:3:231

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