Abstract
Douglas-fir sapwood and heartwood were impregnated with SO2 and steam exploded at three severity levels, and the cellulose-rich, water-insoluble component was enzymatically hydrolyzed. The high-severity conditions resulted in near complete solubilization and some degradation of hemicelluloses and a significant improvement in the efficiency of enzymatic digestibility of the cell ulose component. At lower severity, some of the hemicellulose remained un hydrolyzed, and the cellulose present in the pretreated solids was not readily hydrolyzed. The medium-severity pretreatment conditions proved to be a good compromise because they improved the enzymatic hydrolyzability of the solids and resulted in the recovery of the majority of hemicellulose in a monomeric form within the water-soluble stream. Sapwood-derived wood chips exhibited a higher susceptibility to both pretreatment and hydrolysis and, on steam explosion, formed smaller particles as compared to heartwood-derived wood chips.
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References
Vallander, L. and Eriksson, K. (1985), Biotechnol. Bioeng. 27, 650–659.
Esteghlalian, A., Hashimoto, A. G., Fenske, J., and Penner, M. (1997), Bioresour. Technol. 59, 129–136.
Penner, M., Hashimoto, A. G., Esteghlalian, A., and Fenske, J. (1995) Am. Chem. Soc. Symp. Ser. (647), Fuller, G., McKesn, T. A., and Bills, D. D., eds., ACS, Washington, DC, pp. 12–31.
Saddler, J. N., Mes-Hartree, M., Yu, E. K. C., and Brownell, H. H. (1983), Biotechnol. Bioeng Symp. 13, 225–238.
Sawada, T., Nakamura, Y., Kobayashi, F., Kuwahara, M., and Watanabe, T. (1995), Biotechnol. Bioeng. 48, 719–724.
Szczodrak, J. (1988), Biotechnol. Bioeng. 32, 771–776.
Hahn-Hagerdal, B., Linden, T., Senac, T., and Skoog, K. (1991), Appl. Biochem. Biotechnol. 28/29, 131–144.
Nishikawa, N., Sutcliffe, R., and Saddler, J. N. (1988), Biotechnol. Bioeng. 31, 624–627.
Philippidis, G., Smith, T., and Wyman, C. (1993), Biotechnol. Bioeng. 41, 846–853.
Spindler, D., Wyman, C., and Grohman, K. (1991), Appl. Biochem. Biotechnol. 28/29, 773–786.
Gregg, D. J., Boussaid, A., and Saddler, J. N. (1998), Bioresour. Technol. 63, 7–12.
Mackie, K. L., Brownell, H. H., West, K. L., and Saddler, J. N. (1985), J. Wood Chem. Technol. 5(3), 405–425.
Tengborg, C., Stenberg, K., Galbe, M., Zacchi, G., Larrson, S., Palmqvist, E., and Hahn-Hagerdal, B. (1998), Appl. Biochem. Biotech. 70/72, 3–15.
Ramos, L. P. and Saddler, J. N. (1994), Am. Chem. Soc. Symp. Ser. (566), Himmel, M., Baker, J. and Overend, R., eds. ACS, Washington, DC, pp. 325–341.
Sjostrom, E. (1993), Wood Chemistry, Academic, San Diego.
Boussaid, A., Jarvis, J., Gregg, D. J., and Saddler, J. N. (1997), in The Third Biomass Conference of the Americas, Overend, R. P. and Chornet, E., eds., Montreal, Canada, pp. 873–880.
Stone, J. E., Scallan, A. M., Donefer, E., and Ahlgren, E. (1969), Adv. Chem. Ser. (95), Washington, DC, pp. 219–244.
Allan, G. G., Ko, Y. C., and Ritzenthaler, P. (1991), Tappi 3, 205–212.
Peters, L. E., Walker, L. P., Wilson, D. B., and Irwin, D. C. (1991), Bioresour. Technol. 35, 313–319.
Mooney, C., Mansfield, S., Beatson, R., and Saddler, J. N. (1999), submitted.
Mooney, C., Mansfield, S., Touhy, M., and Saddler, J. N. (1998), Bioresource. Technol. 64, 113–119.
Mansfield, S., Mooney, S., and Saddler, J. N. (1999), Biotech. Prog. 15, 804–816.
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Boussaid, AL., Esteghlalian, A.R., Gregg, D.J. et al. Steam pretreatment of douglas-fir wood chips. Appl Biochem Biotechnol 84, 693–705 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:693
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DOI: https://doi.org/10.1385/ABAB:84-86:1-9:693