Abstract
In this paper, ion-exclusion chromatography with electrospray ionization mass spectrometry was investigated as an alternative method to confirm and determine sugar (glucose, fructose and sucrose) using volatile acidic eluent. Under the positive or negative ionization mode, the ESI-MS provided reasonable signals from positive ion mode as [M+H]+ and [M+NH4]+, or from negative ion mode as [M−H]− and [M+Cl–H]−. However, higher mass spectral intensity for ions was obtained under the positive mode. The separation of target sugars was achieved within 8 min using an eluent containing 0.1% (v/v) acetic acid. Linear plots of peak area versus concentration were obtained over the range 20–5,000 μg L−1. The detection limits of these compounds ranged from 10 to 15 μg L−1. The proposed method was used for the confirmation and quantification of sugars in soft drinks.
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References
Adams MA, Chen ZL, Landman P, Comer TD (2005) Anal Biochem 264:77–84
Jensen MB, Johnson DC (1997) Anal Chem 69:1776–1781. doi:10.1021/ac960828x
Lee YC (1996) J Chromatogr A 720:137–149. doi:10.1016/0021-9673(95)00222-7
Rocklin RD, Clarke AP, Weitzhandler M (1998) Anal Chem 70:1496–1501. doi:10.1021/ac970906w
Cheng J, Jandik P, Avdalovic N (2003) Anal Chem 75:572–579. doi:10.1021/ac0204754
Clarke AP, Jandik J, Rocklin RD, Liu Y, Avdalovic N (1999) Anal Chem 71:2774–2781. doi:10.1021/ac9811524
Yu H, Ding Y, Mou S, Jandik P, Cheng J (2002) J Chromatogr A 966:89–97. doi:10.1016/S0021-9673(02)00739-2
Cai Y, Liua J, Shi Y, Liang L, Mou S (2005) J Chromatogr A 1085:98–103. doi:10.1016/j.chroma.2004.11.100
Fischer K (2002) Anal Chim Acta 465:157–173. doi:10.1016/S0003-2670(02)00204-0
Chen ZL, Adams MA (1999) Anal Chim Acta 386:249–256. doi:10.1016/S0003-2670(99)00009-4
Chen ZL, Glod BK, Adams MA (1998) J Chromatogr A 818:61–68. doi:10.1016/S0021-9673(98)00494-4
Chen ZL, Adams MA (1998) J Liq Chromatogr Relat Technol 21:24352–24445
Buttler T, Gordon L, Marko-Varga G (1993) Anal Chim Acta 279:27–37. doi:10.1016/0003-2670(93)85063-P
Chen ZL, Hibbert DB (1997) J Chromatogr A 766:27–33. doi:10.1016/S0021-9673(96)00971-5
Rosen AL, Hieftje GM (2004) Spectrochim Acta, B At Spectrosc 59:135–146. doi:10.1016/j.sab.2003.09.004
Reemtsma T (2003) J Chromatogr A 1000:477–501. doi:10.1016/S0021-9673(03)00507-7
Bruggink C, Maurer R, Herrmann H, Cavalli S, Hoefler F (2005) J Chromatogr A 1085:104–109. doi:10.1016/j.chroma.2005.03.108
Guignard G, Jouve ML, Bog’eat-Triboulot B, Dreyer E, Hausman JF, Hoffmann L (2005) J Chromatogr A 1085:137–142. doi:10.1016/j.chroma.2005.05.068
Cheng CY, Tsai HR, Chang KC (2006) J Chromatogr A 1119:188–196. doi:10.1016/j.chroma.2005.11.115
Wan ECH, Yu JZ (2006) J Chromatogr A 1107:175–181. doi:10.1016/j.chroma.2005.12.062
Gamoh K, Saitoh H, Wada H (2003) Rapid Commun Mass Spectrom 17:685–689. doi:10.1002/rcm.971
Tanaka K, Fritz JS (1986) J Chromatogr A 361:151–159. doi:10.1016/S0021-9673(01)86902-8
Tanaka K, Ohta K, Fritz JS, Lee YS, Shim SB (1995) J Chromatogr A 706:385–393. doi:10.1016/0021-9673(95)00041-K
Glod BK, Baumann M (2003) J Sep Sci 26:1547–1553. doi:10.1002/jssc.200301559
DIONEX, “Determination of sugar alcohols in confections and fruit juices by high-performance anion-exchange chromatography with pulsed amperometric detection” Application note 87, http://www.dionex.com
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Chen, Z., Jin, X., Wang, Q. et al. Confirmation and Determination of Sugars in Soft Drink Products by IEC with ESI-MS. Chroma 69, 761–764 (2009). https://doi.org/10.1365/s10337-009-0969-3
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DOI: https://doi.org/10.1365/s10337-009-0969-3