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Development and Validation of a Reversed-Phase HPLC Method for the Determination of Deflazacort in Pharmaceutical Dosage Forms

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Abstract

A reversed-phase high-performance liquid chromatographic method has been developed and validated for quantitative determination of deflazacort in tablets and in compounded capsules. Isocratic chromatography was performed on a C18 column with acetonitrile–water 80:20 (v/v) as mobile phase at a flow rate of 1.0 mL min−1. UV detection was at 240 nm. The linearity of the method was good (r > 0.999), as also were intra-day and inter-day precision (RSD <2%) and accuracy (recovery >98%). The method was also validated for specificity and robustness. The results showed the proposed method is suitable for its intended use.

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Authors and Affiliations

  1. Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, CEP 97. 119-900, RS, Brazil

    G. M. Corrêa, L. P. Bellé, L. Bajerski, S. H. M. Borgmann & S. G. Cardoso

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  1. G. M. Corrêa
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  2. L. P. Bellé
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  3. L. Bajerski
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  4. S. H. M. Borgmann
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  5. S. G. Cardoso
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Correspondence to S. G. Cardoso.

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Corrêa, G.M., Bellé, L.P., Bajerski, L. et al. Development and Validation of a Reversed-Phase HPLC Method for the Determination of Deflazacort in Pharmaceutical Dosage Forms. Chroma 65, 591–594 (2007). https://doi.org/10.1365/s10337-007-0205-y

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  • DOI: https://doi.org/10.1365/s10337-007-0205-y

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