Abstract
A high-performance liquid chromatographic (HPLC) method with refractive-index detection has been developed for simultaneous analysis of glycerol, dihydroxyacetone (DHA), 3-hydroxypropionaldehyde (3-HPA), and 1,3-propanediol (1,3-PD), four key substances in the metabolic pathway for production of 1,3-PD from glycerol by microorganism fermentation. The compounds were separated on a 300 mm × 7.8 mm ion-exclusion column with a 65:35 (v/v) mixture of deionized water and acetonitrile, containing 0.0005 M H2SO4, as mobile phase. The flow rate was 0.5 mL min−1. Under these conditions the retention times of 3-HPA, DHA, glycerol, and 1,3-PD were 6.87, 14.63, 16.37, and 18.50 min, respectively. Relative standard deviations and average recoveries were between 0.42 and 0.63% and between 96.7 and 103.1%, respectively; detection limits were between 0.017 and 0.038 g L−1. The method enabled separation of these compounds.
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Acknowledgments
This work was financially supported by the NNSFC (National Natural Science Foundation of China), grant no. 20676048, National “973” Project (no. 2007CB707804), NSFFC (Natural Science Foundation of Fujian, China), grant no. E0510018, and STFFC (Science and Technology Foundation of Fujian, China), grant no. 2003I020. The prophase work was performed at the German Research Center for Biotechnology, Department of Genome Analysis, Mascheroder Weg 1, 38124 Braunschweig, Germany.
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Chen, H., Fang, B. & Hu, Z. Simultaneous HPLC Determination of Four Key Metabolites in the Metabolic Pathway for Production of 1,3-Propanediol from Glycerol. Chroma 65, 629–632 (2007). https://doi.org/10.1365/s10337-007-0193-y
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DOI: https://doi.org/10.1365/s10337-007-0193-y