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Simultaneous Determination of Tetrahydropalmatine, Magnolol, Emodin and Chrysophanol in Chinese Herbal Preparation by RP-HPLC-PDA

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Abstract

A reversed phase high performance liquid chromatography coupled with photo-diode array (RP-HPLC-PDA) detection method was proposed for simultaneous determination of tetrahydropalmatine, magnolol, emodin and chrysophanol in a Chinese herbal preparation (Dan’an mixture). The separation was performed on a Diamonsil™ C18 column (250 × 4.6 mm, 5 μm) with methanol and 0.1% phosphoric acid (88:12, v/v) as the mobile phase at the flow-rate of 0.8 mL min−1. Two detection wavelengths were utilized for the quantitative analysis (209 nm for tetrahydropalmatine and magnolol, and 220 nm for emodin and chrysophanol, respectively). A good linear regression relationship (r ≥ 0.9996) between peak-areas and concentrations was obtained over the range of 0.25–50 μg mL−1 for all the analytes. The spike recoveries, measured at three concentration levels, varied from 90.13 to 102.11%. The method was successfully applied to determine the contents of the four compounds in Dan’an mixture.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (NSFC 20021001) and the Tackle Key Projects of Gansu, China (2GS042-A43-014-12).

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Authors and Affiliations

  1. School of Pharmacy, Lanzhou University, Lanzhou, 730000, People’s Republic of China

    Yan-Bin Shi

  2. State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000, People’s Republic of China

    Yan-Bin Shi & Yan-Ping Shi

  3. The First Hospital of Lanzhou city, Lanzhou, 730000, People’s Republic of China

    Yan-Biao Yang & Guang Feng

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  1. Yan-Bin Shi
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  2. Yan-Ping Shi
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  3. Yan-Biao Yang
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Correspondence to Yan-Ping Shi.

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Shi, YB., Shi, YP., Yang, YB. et al. Simultaneous Determination of Tetrahydropalmatine, Magnolol, Emodin and Chrysophanol in Chinese Herbal Preparation by RP-HPLC-PDA. Chroma 65, 601–606 (2007). https://doi.org/10.1365/s10337-007-0191-0

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  • DOI: https://doi.org/10.1365/s10337-007-0191-0

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