Abstract
Novel procedures for the determination of florfenicol in freshwater, sediments and bryophyte Fontinalis antipyretica, using reversed-phase high-performance liquid chromatography are described. Liquid chromatography was performed on a 5 µm PuroSpher RP-18E® column using methanol and 0.05 M phosphate buffer (18/82 v/v, pH 7.3) as mobile phase (0.8 ml min−1) and fluorescence detection (excitation wavelength 265 nm and emission wavelength 295 nm). Florfenicol was determined in centrifuged freshwater samples. Florfenicol was extracted from sediments and bryophytes samples by using a solid-liquid extraction step followed by a solid phase extraction step. Linearity was confirmed over the concentration range 25–1000 ng mL−1 water and 50-1000 ng g−1 sediment or bryophyte. Limits of detection and quantitation were 8 and 25 ng mL−1 water and 17 and 50 ng g−1 sediment or bryophyte respectively. Mean extraction recoveries of florfenicol from sediments and bryophyte were from 85.9 to 109.1%.
Similar content being viewed by others
References
Ho SP, Hsu TY, Chen MH, Wang WS (2000) J Vet Med Sci 62(5):479–485
Martinsen B, Horsberg TE, Varma KJ, Sams R (1993) Aquaculture 112:1–11
Delépée R, Pouliquen H, Le Bris H (2004) Sci Total Environ 322:243–253
Nagata T (1995) Chemical analysis of chloramphenicols. In: Okaé H, Nakazawa H, Harada K, Macneil JD (eds) Chemical analysis of antibiotics in agriculture. AOAC International, Arlington, VA, USA, pp. 207–234
Nagata T, Oka H (1996) J. Agric Food Chem 44(5):1280–1284
Hormazabal V, Steffenak I, Yndestad M (1993) J Chromatogr B 616(1):161–165
De Craene BA, Deprez P, D’Haese E, Nelis HJ, Van den Bossche W, De Leenheer AP (1997) Antimicrob Agents Chemother 41(9):1991–1995
Vue C, Schmidt LJ, Stehly GR, Gingerich WH (2002) J Chromatogr B 780:111–117
Stamper MA, Papich MG, Lewbart GA, May SB, Plummer DD, Stoskopf MK (2003) J Zoo Wildlife Med. 34(1):3–8
Hillaert S, Van den Bossche W (2004) J Pharm Biomed Anal 36(3):437–440
Hormazabal V, Steffenak I, Yndestad M (1996) J Chromatogr A 724:364–366
Cooper AD, Tarbin JA, Farrington WHH, Shearer G (1998) Food Addit Contam 15(6):637–644
Caniou I, Nikolaides E, Tsoukali H, Stratis JA, Zachariadis GA (1995) J Liq Chromatogr Related Technol 18(17):3519–3527
Roudaut B (1996) J Liq Chromatogr Related Technol 19(7):1097–1105
Ramos M, Reuvers T, Aranda A, Gomez J (1994) J Liq Chromatogr 17(2):385–401
Delépée R, Pouliquen H (2002) J Chromatogr B 775:89–95
Delépée R, Pouliquen H (2003) Anal Chim Acta 475:117–123
Di Pietra AM, Piazza V, Andrisano V, Cavrini V (1995) J Liq Chromatogr Related Technol 18(17):3529–3543
Pouliquen H, Le Bris H, Pinault L (1994b) Quim Anal (Barcelona), 13(Suppl 1):S109–S113
Chapuzet E, Mercier N (1997) S.T.P. Pharma Pratiques 7:169–194
Lang JR, Bolton S (1991) J Pharm Biomed Anal 9:435–442
Pouliquen H, Le Bris H, Pinault L (1992) Mar Ecol Progr Ser 89:93–98
Pouliquen H, Le Bris H, Pinault L (1994a) J Liq Chromatogr 17(4):929–945
Author information
Authors and Affiliations
Corresponding author
Additional information
Revised : 6 June and 7 July 2005
Rights and permissions
About this article
Cite this article
Pouliquen, H., Morvan, M.L. Determination of Florfenicol in Freshwater, Sediments and Bryophyte Fontinalis antipyretica by HPLC with Fluorescence Detection. Chroma 62, 225–231 (2005). https://doi.org/10.1365/s10337-005-0631-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-005-0631-7