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Determination of Florfenicol in Freshwater, Sediments and Bryophyte Fontinalis antipyretica by HPLC with Fluorescence Detection

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Abstract

Novel procedures for the determination of florfenicol in freshwater, sediments and bryophyte Fontinalis antipyretica, using reversed-phase high-performance liquid chromatography are described. Liquid chromatography was performed on a 5 µm PuroSpher RP-18E® column using methanol and 0.05 M phosphate buffer (18/82 v/v, pH 7.3) as mobile phase (0.8 ml min−1) and fluorescence detection (excitation wavelength 265 nm and emission wavelength 295 nm). Florfenicol was determined in centrifuged freshwater samples. Florfenicol was extracted from sediments and bryophytes samples by using a solid-liquid extraction step followed by a solid phase extraction step. Linearity was confirmed over the concentration range 25–1000 ng mL−1 water and 50-1000 ng g−1 sediment or bryophyte. Limits of detection and quantitation were 8 and 25 ng mL−1 water and 17 and 50 ng g−1 sediment or bryophyte respectively. Mean extraction recoveries of florfenicol from sediments and bryophyte were from 85.9 to 109.1%.

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Correspondence to H. Pouliquen.

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Revised : 6 June and 7 July 2005

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Pouliquen, H., Morvan, M.L. Determination of Florfenicol in Freshwater, Sediments and Bryophyte Fontinalis antipyretica by HPLC with Fluorescence Detection. Chroma 62, 225–231 (2005). https://doi.org/10.1365/s10337-005-0631-7

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  • DOI: https://doi.org/10.1365/s10337-005-0631-7

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